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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB300-284 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-284, RRID:AB_2147428
- Product name
- Mouse Monoclonal MYH6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. This antibody recognizes alpha and beta type heavy chains of light meromyosin fragments (LMM) of cardiac myosin heavy chains. It also reacts with LMM fragments of cardiac myosin and beta heavy chain of slow human skeletal muscle. The antibody does not crossreact with human myosin light chains.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- LYOPH
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The E3 SUMO ligase Nse2 regulates sumoylation and nuclear-to-cytoplasmic translocation of skNAC-Smyd1 in myogenesis.
Overexpression of the skNAC gene in human rhabdomyosarcoma cells enhances their differentiation potential and inhibits tumor cell growth and spreading.
Decorin activates Akt downstream of IGF-IR and promotes myoblast differentiation.
Injection of porous polycaprolactone beads containing autologous myoblasts in a dog model of fecal incontinence.
Downregulation in GATA4 and Downstream Structural and Contractile Genes in the db/db Mouse Heart.
Skeletal muscle-specific variant of nascent polypeptide associated complex alpha (skNAC): implications for a specific role in mammalian myoblast differentiation.
Electrophoretic mobility of cardiac myosin heavy chain isoforms revisited: application of MALDI TOF/TOF analysis.
Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart.
Berkholz J, Michalick L, Munz B
Journal of cell science 2014 Sep 1;127(Pt 17):3794-804
Journal of cell science 2014 Sep 1;127(Pt 17):3794-804
Overexpression of the skNAC gene in human rhabdomyosarcoma cells enhances their differentiation potential and inhibits tumor cell growth and spreading.
Berkholz J, Kuzyniak W, Hoepfner M, Munz B
Clinical & experimental metastasis 2014 Dec;31(8):869-79
Clinical & experimental metastasis 2014 Dec;31(8):869-79
Decorin activates Akt downstream of IGF-IR and promotes myoblast differentiation.
Suzuki K, Kishioka Y, Wakamatsu J, Nishimura T
Animal science journal = Nihon chikusan Gakkaiho 2013 Sep;84(9):669-74
Animal science journal = Nihon chikusan Gakkaiho 2013 Sep;84(9):669-74
Injection of porous polycaprolactone beads containing autologous myoblasts in a dog model of fecal incontinence.
Kang SB, Lee HS, Lim JY, Oh SH, Kim SJ, Hong SM, Jang JH, Cho JE, Lee SM, Lee JH
Journal of the Korean Surgical Society 2013 Apr;84(4):216-24
Journal of the Korean Surgical Society 2013 Apr;84(4):216-24
Downregulation in GATA4 and Downstream Structural and Contractile Genes in the db/db Mouse Heart.
Broderick TL, Jankowski M, Wang D, Danalache BA, Parrott CR, Gutkowska J
ISRN endocrinology 2012;2012:736860
ISRN endocrinology 2012;2012:736860
Skeletal muscle-specific variant of nascent polypeptide associated complex alpha (skNAC): implications for a specific role in mammalian myoblast differentiation.
Berger F, Berkholz J, Breustedt T, Ploen D, Munz B
European journal of cell biology 2012 Feb;91(2):150-5
European journal of cell biology 2012 Feb;91(2):150-5
Electrophoretic mobility of cardiac myosin heavy chain isoforms revisited: application of MALDI TOF/TOF analysis.
Arnostova P, Jedelsky PL, Soukup T, Zurmanova J
Journal of biomedicine & biotechnology 2011;2011:634253
Journal of biomedicine & biotechnology 2011;2011:634253
Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart.
Ott HC, Matthiesen TS, Goh SK, Black LD, Kren SM, Netoff TI, Taylor DA
Nature medicine 2008 Feb;14(2):213-21
Nature medicine 2008 Feb;14(2):213-21
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MYH6 Antibody (3-48) [NB300-284] - analysis of total cardiac myosin expression in tissue lysates of rat heart (lanes 1-5), liver (lanes 6-7), and lung (lanes 8-9). Increasing amounts of total protein were loaded as follow: lanes 1, 6 and 8: 0 ug; lane 2: 50 ug; lanes 3 , 7 and 9: 250 ug; lane 5: 500 ug. (anti-cMHC was used at 1:500; secondary antibody: Goat anti-mouse IgG Fc-HRP; 1:5000)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MYH6 Antibody (3-48) [NB300-284] - Western blot immunodetection of cardiac MyHC isoforms from left ventricle (LV) homogenates and extracts of adult euthyroid (EU), hypothyroid (HY), and hyperthyroid (TH) Lewis rats. The membranes were stained by NB300-284 antibody recognizing both MyHCalpha and MyHCbeta isoforms. Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/bmri/2011/634253/), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MYH6 Antibody (3-48) [NB300-284] - Formaldehyde-fixed paraffin-embedded (FFPE) Tissue Slides. Human heart ventricle following immunostaining with cMHC 3-48 and irrelevant NeuAc monoclonal antibody at low microscopical magnification.
