Antibody data
- Antibody Data
- Antigen structure
- References [22]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [11]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 14-4502-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha Tubulin Monoclonal Antibody (DM1A), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody DM1a recognizes the 50 kDa cytoskeletal protein alpha tubulin in a variety of species (human, mouse, rat, monkey, dog, pig, bovine, goat, hamster, guinea pig, kangaroo, amphibians, sea urchin, yeast and tobacco plants). Tubulin, the major component of microtubules, is a dimeric protein consisting of an alpha and beta subunit. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation resulting in assembly (polymerization) or disassembly (depolymerization). The dynamic nature of microtubules is most evident in the mitotic apparatus. The DM1a antibody recognizes the C-terminal end of the alpha tubulin isoform (amino acids 426-430).
- Antibody clone number
- DM1A
- Concentration
- 0.5 mg/mL
Submitted references Engrailed 1 coordinates cytoskeletal reorganization to induce myofibroblast differentiation.
Targeting EML4-ALK gene fusion variant 3 in thyroid cancer.
Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.
Androstano-arylpyrimidines: Novel small molecule inhibitors of MDR1 for sensitizing multidrug-resistant breast cancer cells.
Loss of the Nuclear Protein RTF2 Enhances Influenza Virus Replication.
The extracellular domain of angulin-1 and palmitoylation of its cytoplasmic region are required for angulin-1 assembly at tricellular contacts.
Metabolic reprogramming related to whole-chromosome instability in models for Hürthle cell carcinoma.
PKCγ-Mediated Phosphorylation of CRMP2 Regulates Dendritic Outgrowth in Cerebellar Purkinje Cells.
GATA factor-regulated solute carrier ensemble reveals a nucleoside transporter-dependent differentiation mechanism.
Endoplasmic reticulum stress: major player in size-dependent inhibition of P-glycoprotein by silver nanoparticles in multidrug-resistant breast cancer cells.
Palmitic acid induces guanylin gene expression through the Toll-like receptor 4/nuclear factor-κB pathway in rat macrophages.
AAGAB Controls AP2 Adaptor Assembly in Clathrin-Mediated Endocytosis.
MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins.
Multiple Levels of Control Determine How E4bp4/Nfil3 Regulates NK Cell Development.
RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis.
The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1α prolyl hydroxylation by regulating cellular iron levels.
Do mechanical strain and TNF-α interact to amplify pro-inflammatory cytokine production in human annulus fibrosus cells?
Disruption of tumour-host communication by downregulation of LFA-1 reduces COX-2 and e-NOS expression and inhibits brain metastasis growth.
Comparative Haploid Genetic Screens Reveal Divergent Pathways in the Biogenesis and Trafficking of Glycophosphatidylinositol-Anchored Proteins.
IP-10/CXCL10 induction in human pancreatic cancer stroma influences lymphocytes recruitment and correlates with poor survival.
Loss of PTEN induces microtentacles through PI3K-independent activation of cofilin.
Chikungunya virus nsP3 blocks stress granule assembly by recruitment of G3BP into cytoplasmic foci.
Györfi AH, Matei AE, Fuchs M, Liang C, Rigau AR, Hong X, Zhu H, Luber M, Bergmann C, Dees C, Ludolph I, Horch RE, Distler O, Wang J, Bengsch B, Schett G, Kunz M, Distler JHW
The Journal of experimental medicine 2021 Sep 6;218(9)
The Journal of experimental medicine 2021 Sep 6;218(9)
Targeting EML4-ALK gene fusion variant 3 in thyroid cancer.
Aydemirli MD, van Eendenburg JDH, van Wezel T, Oosting J, Corver WE, Kapiteijn E, Morreau H
Endocrine-related cancer 2021 May 11;28(6):377-389
Endocrine-related cancer 2021 May 11;28(6):377-389
Rapid degradation of GRASP55 and GRASP65 reveals their immediate impact on the Golgi structure.
Zhang Y, Seemann J
The Journal of cell biology 2021 Jan 4;220(1)
The Journal of cell biology 2021 Jan 4;220(1)
Androstano-arylpyrimidines: Novel small molecule inhibitors of MDR1 for sensitizing multidrug-resistant breast cancer cells.
Gopisetty MK, Adamecz DI, Nagy FI, Baji Á, Lathira V, Szabó MR, Gáspár R, Csont T, Frank É, Kiricsi M
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2021 Jan 1;156:105587
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2021 Jan 1;156:105587
Loss of the Nuclear Protein RTF2 Enhances Influenza Virus Replication.
Chia BS, Li B, Cui A, Eisenhaure T, Raychowdhury R, Lieb D, Hacohen N
Journal of virology 2020 Oct 27;94(22)
Journal of virology 2020 Oct 27;94(22)
The extracellular domain of angulin-1 and palmitoylation of its cytoplasmic region are required for angulin-1 assembly at tricellular contacts.
Oda Y, Sugawara T, Fukata Y, Izumi Y, Otani T, Higashi T, Fukata M, Furuse M
The Journal of biological chemistry 2020 Mar 27;295(13):4289-4302
The Journal of biological chemistry 2020 Mar 27;295(13):4289-4302
Metabolic reprogramming related to whole-chromosome instability in models for Hürthle cell carcinoma.
Addie RD, Kostidis S, Corver WE, Oosting J, Aminzadeh-Gohari S, Feichtinger RG, Kofler B, Aydemirli MD, Giera M, Morreau H
Scientific reports 2020 Jun 12;10(1):9578
Scientific reports 2020 Jun 12;10(1):9578
PKCγ-Mediated Phosphorylation of CRMP2 Regulates Dendritic Outgrowth in Cerebellar Purkinje Cells.
Winkler SC, Shimobayashi E, Kapfhammer JP
Molecular neurobiology 2020 Dec;57(12):5150-5166
Molecular neurobiology 2020 Dec;57(12):5150-5166
GATA factor-regulated solute carrier ensemble reveals a nucleoside transporter-dependent differentiation mechanism.
Zwifelhofer NM, Cai X, Liao R, Mao B, Conn DJ, Mehta C, Keles S, Xia Y, Bresnick EH
PLoS genetics 2020 Dec;16(12):e1009286
PLoS genetics 2020 Dec;16(12):e1009286
Endoplasmic reticulum stress: major player in size-dependent inhibition of P-glycoprotein by silver nanoparticles in multidrug-resistant breast cancer cells.
Gopisetty MK, Kovács D, Igaz N, Rónavári A, Bélteky P, Rázga Z, Venglovecz V, Csoboz B, Boros IM, Kónya Z, Kiricsi M
Journal of nanobiotechnology 2019 Jan 22;17(1):9
Journal of nanobiotechnology 2019 Jan 22;17(1):9
Palmitic acid induces guanylin gene expression through the Toll-like receptor 4/nuclear factor-κB pathway in rat macrophages.
Akieda-Asai S, Ma H, Date Y
American journal of physiology. Cell physiology 2019 Dec 1;317(6):C1239-C1246
American journal of physiology. Cell physiology 2019 Dec 1;317(6):C1239-C1246
AAGAB Controls AP2 Adaptor Assembly in Clathrin-Mediated Endocytosis.
Gulbranson DR, Crisman L, Lee M, Ouyang Y, Menasche BL, Demmitt BA, Wan C, Nomura T, Ye Y, Yu H, Shen J
Developmental cell 2019 Aug 19;50(4):436-446.e5
Developmental cell 2019 Aug 19;50(4):436-446.e5
MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins.
Stefanovic-Barrett S, Dickson AS, Burr SP, Williamson JC, Lobb IT, van den Boomen DJ, Lehner PJ, Nathan JA
EMBO reports 2018 May;19(5)
EMBO reports 2018 May;19(5)
Multiple Levels of Control Determine How E4bp4/Nfil3 Regulates NK Cell Development.
Kostrzewski T, Borg AJ, Meng Y, Filipovic I, Male V, Wack A, DiMaggio PA, Brady HJM
Journal of immunology (Baltimore, Md. : 1950) 2018 Feb 15;200(4):1370-1381
Journal of immunology (Baltimore, Md. : 1950) 2018 Feb 15;200(4):1370-1381
RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis.
Gulbranson DR, Davis EM, Demmitt BA, Ouyang Y, Ye Y, Yu H, Shen J
Proceedings of the National Academy of Sciences of the United States of America 2017 Sep 26;114(39):E8224-E8233
Proceedings of the National Academy of Sciences of the United States of America 2017 Sep 26;114(39):E8224-E8233
The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1α prolyl hydroxylation by regulating cellular iron levels.
Miles AL, Burr SP, Grice GL, Nathan JA
eLife 2017 Mar 15;6
eLife 2017 Mar 15;6
Do mechanical strain and TNF-α interact to amplify pro-inflammatory cytokine production in human annulus fibrosus cells?
Likhitpanichkul M, Torre OM, Gruen J, Walter BA, Hecht AC, Iatridis JC
Journal of biomechanics 2016 May 3;49(7):1214-1220
Journal of biomechanics 2016 May 3;49(7):1214-1220
Disruption of tumour-host communication by downregulation of LFA-1 reduces COX-2 and e-NOS expression and inhibits brain metastasis growth.
Soto MS, O'Brien ER, Andreou K, Scrace SF, Zakaria R, Jenkinson MD, O'Neill E, Sibson NR
Oncotarget 2016 Aug 9;7(32):52375-52391
Oncotarget 2016 Aug 9;7(32):52375-52391
Comparative Haploid Genetic Screens Reveal Divergent Pathways in the Biogenesis and Trafficking of Glycophosphatidylinositol-Anchored Proteins.
Davis EM, Kim J, Menasche BL, Sheppard J, Liu X, Tan AC, Shen J
Cell reports 2015 Jun 23;11(11):1727-36
Cell reports 2015 Jun 23;11(11):1727-36
IP-10/CXCL10 induction in human pancreatic cancer stroma influences lymphocytes recruitment and correlates with poor survival.
Lunardi S, Jamieson NB, Lim SY, Griffiths KL, Carvalho-Gaspar M, Al-Assar O, Yameen S, Carter RC, McKay CJ, Spoletini G, D'Ugo S, Silva MA, Sansom OJ, Janssen KP, Muschel RJ, Brunner TB
Oncotarget 2014 Nov 30;5(22):11064-80
Oncotarget 2014 Nov 30;5(22):11064-80
Loss of PTEN induces microtentacles through PI3K-independent activation of cofilin.
Vitolo MI, Boggs AE, Whipple RA, Yoon JR, Thompson K, Matrone MA, Cho EH, Balzer EM, Martin SS
Oncogene 2013 Apr 25;32(17):2200-10
Oncogene 2013 Apr 25;32(17):2200-10
Chikungunya virus nsP3 blocks stress granule assembly by recruitment of G3BP into cytoplasmic foci.
Fros JJ, Domeradzka NE, Baggen J, Geertsema C, Flipse J, Vlak JM, Pijlman GP
Journal of virology 2012 Oct;86(19):10873-9
Journal of virology 2012 Oct;86(19):10873-9
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoblotting of reduced lysates from Jurkat T cells probed with 2 µg/mL DM1A.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of alpha tubulin (green) in a BHP 2-7 thyroid cancer anaphase cell cultured in complete medium on a glass slide. Cells were fixed with 4% phosphate-buffered paraformaldehyde for 30 min at 37C, permeabilized with 100% ice-cold methanol for 20 min. and blocked with PBS/1% BSA/0.5% Tween 20 at 37C for 30 min. Cells were stained with monoclonal alpha tubulin antibody (Product # 14-4502-82) at a dilution of 1:200 in PBS/1% BSA/0.1% Tween 20 for 60 min at 37C., and then incubated with goat anti-mouse IgG1 AF488 (Product # A-21121) at a dilution of 1:200 for 60 min. at 37C (panel b: green). HEC1 (panel a: red) was stained simultaneously with monoclonal HEC1 mouse IgG1 antibody (Product # 14-4502-82) followed by goat anti-mouseIgG2a AF594 (1:200, Cat # A-21135). DNA was stained with Hoechst33342 (panel c: blue). Panels d shows a merged image. Images were taken with a 40x immersion oil objective (for more information: Corver et al., Endocr Relat Cancer. 2018 Jan;25(1):83-97). Data courtesy of Antibody Data Exchange Program.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Functional effects of IP-10 on PCCs (A) Expression of CXCR3 in PCCs, PSCs and HEK293-EBNA/CXCR3-B analyzed by Western Blot. alpha-tubulin was used as loading control. (B) Microphotographs of PCCs migrated through the Boyden chamber in response to 0.5% FBS or IP-10 (24 hr). (C-D) Viability of PCCs after IP-10 addition determined using the MTT assay. Low/high refers to cell seeding density. Data are representative of two/three independent experiments. Data in the histograms are presented as mean +- SD.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Intracellular levels of glutathione and ROS in the TCCLs, XTC.UC1, FTC-236, SW579 and BHP2-7; ( a ) ratio of reduced to oxidized glutathione, GSH/GSSG; ( b ) intracellular concentrations of GSH, GSSG and the glutathione precursors, glutamate and glycine; ( c ). uptake of glutathione precursors cysteine and serine from the culture medium (expressed as relative change, i.e. mumol/h/10 6 cells of each metabolite in each cell line relative to the mean); ( d ). intracellular levels of GSH, GSSG and serine under normal culture condition (glucose) and under glucose to galactose exchange for 6 and 24 h; ( e ). Flow cytometric analysis of ROS: left panel, hydroxyl radicals detected by CellROX, right panel superoxide radicals detected by MitoSOX. Background fluorescence (left half each panel) and after incubation with CellROX or MitoSOX, respectively (right half). Fluorescence was analysed after cells were either untreated, NAC-treated, AMA-treated or AMA + NAC-treated. f. SOD2 immunoblotting (green signal). alpha-Tubulin was used as internal reference (red signal). A short treatment with 100 uM AMA did not affect protein expression. Left lane, molecular weight markers. Top and bottom of the image were cropped. For full-length gel, see end Supplementary Information.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIG 3 RTF2 localizes to the nucleus. (A) Immunofluorescence of WT A549 cells and two distinct clones of RTF2-KO cells. (B) Biochemical fractionation of WT A549 cells into cytosolic and nuclear fractions. Cells were first suspended in HEPES-sucrose-Ficoll (HSF) buffer containing digitonin to extract the cytosolic proteins and washed once with HSF buffer, before lysing the nuclear pellet in RIPA buffer to release the nuclear proteins. TATA-binding protein (TBP), a nuclear protein, and tubulin, a cytosolic protein, were included as controls for the fractionation protocol. (C) Live-cell imaging of cells expressing either mCherry or the RTF2-mCherry fusion protein. WT A549 (top) and RTF2-KO cells (bottom) were transduced with lentiviruses that encode either FLAG-mCherry or RTF2-FLAG-mCherry and cultured for a week, before they were visualized under a wide-field epifluorescence microscope.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 10.1371/journal.pgen.1009286.g009 Fig 9 GATA factor regulatory circuit links Slc29a1 with adenosine mechanisms. (A) qRT-PCR analysis of Slc29a1 (left) and Gata2 (right) mRNA from Gata2 -77 +/+ and -77 -/- HSPCs isolated from E14.5 fetal liver (n = 10). (B) Representative Western blot analysis to detect Slc29a1 protein in Gata2 -77 +/+ and -77 -/- murine HSPCs isolated from E14.5 fetal liver (left). Quantification of relative band intensities from the immunoblots (n = 10; right). Tubulin served as a loading control. (C) qRT-PCR analysis of Slc29a1 (top) or Adk (bottom) mRNA isolated from untreated vs beta-estradiol-treated (12, 24, or 48 hours) G1E-ER-GATA1 cells. (D) Representative Western blot analysis to detect Slc29a1 and Adk protein levels in untreated vs beta-estradiol-treated (12, 24, or 48 hours) G1E-ER-GATA1 cells (left). Quantification of relative band intensities from the immunoblots (right). Tubulin served as a loading control. Statistical significance was determined by comparing values at 12, 24, or 48-hour times to those from untreated cells (0 hour). Data are from 4 separate experiments (n = 9). (E) ChIP-seq profiles of GATA1 occupancy at human (GEO GSE32491; PBDE) ADK and murine (GEO GSE30142; Ter119+ erythroblast) Adk loci [, ]. The underlined sequences denote WGATAR motifs. (F) AMP and ATP concentrations quantified by a luciferase-based assay in primary erythroblasts infected with shRNA targeting luciferase or Slc29a1 (left two panels). AMP/ATP ratio calculat
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Quantitative, multiplexed near-infrared fluorescent Western blotting. In the Western blot, the expression of proteins and phosphorylated proteins ((p)ALK as the fusion protein of EML4 or NPM1 with conformable molecular sizes, (p)STAT, (p)AKT, (p)ERK and household protein control alpha-Tubulin) for the cancer cell lines JVE404, NCI-H2228, BHP 2-7 and Karpas-299 are shown in the treated conditions of DMSO control, crizotinib (30, 100, 300 nM) and lorlatinib (30, 100, 300 nM), respectively. alphaTub, alpha-Tubulin; CR, crizotinib; LO, lorlatinib.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 Interaction of CRMP2 and PKCgamma. a Immunoblot showing the co-immunoprecipitation of CRMP2 and PKCgamma from cerebellar lysates. Proteins were detected with the indicated antibodies. Normal rabbit IgG was used as a negative control. b HeLa cells were probed with alpha-PKCgamma and alpha-CRMP2 antibody and the interaction detected via Duolink proximity ligation assay (top row); alpha-CRMP2 and alpha-alpha-tubulin antibodies were used as a positive control (middle row) and primary antibodies were omitted as a negative control (bottom row). Blue, nuclei stained with DAPI; red, PLA signal. Scale bar = 20 mum