PA1-32416

antibody from Invitrogen Antibodies

Targeting: ACTB

Antibody data

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Validation data

Product number: PA1-32416 - Provider product page
Provider: Invitrogen Antibodies
Product name: beta Actin Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Guinea Pig, Porcine
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: 4° C, do not freeze

ACTB protein structure - PA1-32416 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CTB & CTG1 in 20 µg of NIH-3T3 lysates using a CTB & CTG1 polyclonal antibody (Product # PA1-32416) at a dilution of 1:15,000 (lane 1), 1:10,000 dilution (lane 2) and 1:15,000 dilution (lane 3) followed by ECL detection at an exposure time of 30 seconds.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CTB & CTG1 in RCC4 whole cell lysate using a CTB & CTG1 polyclonal antibody (Product # PA1-32416).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CTB & CTG1 in HepG2 whole cell lysate using a CTB & CTG1 polyclonal antibody (Product # PA1-32416).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CTB & CTG1 in 5 µg of nuclear extract from mouse bone marrow derived macrophages (lane 1) and 10 µg of cytoplasmic extract from mouse bone marrow derived macrophages (lane 2), using a CTB & CTG1 polyclonal antibody (Product # PA1-32416).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Actin alpha/gamma was performed by loading HepG2 cell lysate. Proteins were transferred to a membrane and probed with a Actin alpha/gamma Polyclonal Antibody (Product # PA1-32416).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of beta Actin was performed by loading 50 µg of HeLa, 293T, Jurkat, TCMK-1, and NIH3T3 cell lysates in NETN lysis buffer. Proteins were transferred to a membrane and probed with a beta Actin Polyclonal Antibody (Product # PA1-32416) at a dilution of 1 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of beta Actin was performed by loading 20 µg of NIH-3T3 cell lysates. Proteins were transferred to a membrane and probed with a beta Actin Polyclonal Antibody (Product # PA1-32416) at dilutions of Lane 1: 1:15,000, Lane 2: 1:10,000, Lane 3: 1:15,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of beta Actin was performed by loading HeLa cell lysate. Proteins were transferred to a membrane and probed with a beta Actin Polyclonal Antibody (Product # PA1-32416).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of beta Actin was performed by loading HepG2 cell lysate. Proteins were transferred to a membrane and probed with a beta Actin Polyclonal Antibody (Product # PA1-32416).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of beta Actin was performed by loading RCC4 cell lysate. Proteins were transferred to a membrane and probed with a beta Actin Polyclonal Antibody (Product # PA1-32416).