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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-36780 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 60 kDa.
- Concentration
- 1 mg/mL
Submitted references Crocin Inhibits Oxidative Stress and Pro-inflammatory Response of Microglial Cells Associated with Diabetic Retinopathy Through the Activation of PI3K/Akt Signaling Pathway.
Yang X, Huo F, Liu B, Liu J, Chen T, Li J, Zhu Z, Lv B
Journal of molecular neuroscience : MN 2017 Apr;61(4):581-589
Journal of molecular neuroscience : MN 2017 Apr;61(4):581-589
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-AKT1+AKT2+AKT3 pTyr315 using Phospho-AKT1+AKT2+AKT3 pTyr315 polyclonal antibody (Product # PA5-36780) at a dilution of 1:500. Lane 1: MCF-7 cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 2: Raw264.7 cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 3: H9C2 cell lysate treated with EGF (0.1 ng/mL, 30 min).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) in paraffin-embedded human kidney carcinoma tissue (cytoplasm, nucleus and membrane staining) and negative control (right, with PBS only). Samples were incubated with Phospho-AKT Pan (Tyr315, Tyr316, Tyr312) polyclonal antibody (Product # PA5-36780) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
