PA5-35893
antibody from Invitrogen Antibodies
Targeting: H2AC11
H2A.1b, H2A/p, H2AFP, HIST1H2AG, pH2A/f
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-35893 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Histone H2A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: MCF-7, MDA-MB-231.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Histone H2A using A) 30 µg MCF-7 whole cell extract and B) 30 µg MDA-MB-231 whole cell extract. Samples were loaded onto a 15% SDS-PAGE gel and probed with a Histone H2A polyclonal antibody (Product # PA5-35893) at a dilution of 1:10000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Histone H2A Polyclonal Antibody (Product # PA5-35893). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A Polyclonal Antibody (Product # PA5-35893) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2A Polyclonal Antibody detects Histone H2A protein at nucleus on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Histone H2A Polyclonal Antibody (Product # PA5-35893) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2A Polyclonal Antibody detects Histone H2A protein at nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Histone H2A Polyclonal Antibody (Product # PA5-35893) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2A Polyclonal Antibody detects Histone H2A protein at nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Histone H2A Polyclonal Antibody (Product # PA5-35893) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2A Polyclonal Antibody detects Histone H2A protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Histone H2A Polyclonal Antibody (Product # PA5-35893) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.