Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA1-30293 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EphA4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: NCI-H460 cell lysate. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Ephrin A4 in 293 cell lysates (2 µg/lane) either non-transfected (Lane 1) or transiently transfected with the EPHA4 gene (Lane 2) using an Ephrin A4 polyclonal antibody (Product # PA1-30293).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Ephrin A4 was performed by loading 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA4 (Lane 2). Proteins were transferred to a membrane and probed with a Ephrin A4 Polyclonal Antibody (Product # PA1-30293).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Ephrin A4 was performed by loading 20 µg per lane of HeLa, HUVEC cell lysates. Proteins were transferred to a membrane and probed with a Ephrin A4 Polyclonal Antibody (Product # PA1-30293) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Ephrin A4 was performed by loading 20 µg per lane of HeLa, NCI-H460, HUVEC cell lysates. Proteins were transferred to a membrane and probed with a Ephrin A4 Polyclonal Antibody (Product # PA1-30293) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Ephrin A4 was performed by loading 35 µg per lane of HeLa, NCI-H460, NIH3T3 cell and human ovary tissue lysates. Proteins were transferred to a membrane and probed with a Ephrin A4 Polyclonal Antibody (Product # PA1-30293) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Ephrin A4 was performed by loading 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA4 (Lane 2). Proteins were transferred to a membrane and probed with a Ephrin A4 Polyclonal Antibody (Product # PA1-30293).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Ephrin A4 was performed by loading NCI-H460 cell lysate. Proteins were transferred to a membrane and probed with a Ephrin A4 Polyclonal Antibody (Product # PA1-30293).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Ephrin A4 in human breast carcinoma tissue using Ephrin A4 Polyclonal Antibody (Product # PA1-30293).