720260

antibody from Invitrogen Antibodies

Targeting: AMY2B AMY2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

720260

Provider

Invitrogen Antibodies

Product name

Pancreatic Amylase Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with Monkey, Dog, Cat

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on Whole cell extracts (30 µg lysate) of MOLT4 (Lane 1), MOLT4 (Condition media) (Lane 2), Raji (Lane 3), Raji (Condition media) (Lane 4), HL-60 (Lane 5), HL-60 (Condition media) (Lane 6), K-562 (Lane 7) and K-562 (Condition media) (Lane 8). The blots were probed with Anti-alpha-amylase Rabbit Polyclonal Antibody (Product # 720260, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 58 kDa in cell lines and 200 kDa in condition media band corresponding to Vitronectin was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).