- MA5-13473 - Invitrogen Antibodies CD8B antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Formalin-fixed, paraffin-embedded human tonsil stained with CD8 antibody using peroxidase-conjugate and DAB chromogen. Note membrane staining of T cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Flow cytometry analysis of CD8 in THP-1 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD8 monoclonal antibody (Product # MA5-13473) at a dilution of 1:2 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 2 Immuno-FISH and CD8+ T-cell infiltrate in primary vs. metastatic samples. a iFISH images of one area of the primary tumor and brain and liver metastases of case 15. Scale bar 100 mm. b Dot plot depicting relative frequencies of the four different cell types in primary and metastatic samples. Error bars, S.E.M. c Immunofluorescence analysis of CD8, GranzymeB (GZMB), and pSTAT3. Images are a montage of nine fields captured from one area of the tissue. Scale bar 100 mm. d , e Graph depicting numbers of CD8 + T cells per montage ( d ) and fraction of GZMB + CD8 + T cells ( e ). Significance of the difference between primary and metastatic samples was calculated using the Wilcoxon rank-sum test. f Correlation analysis between the number of infiltrating CD8 + T cells and the relative frequencies of specific cell populations in primary and metastatic samples. g Correlation analysis between number of infiltrating CD8 + T cells and the Shannon index of diversity in all, primary and metastatic samples. Gray area, 95% confidence interval. Sample sizes were n = 16 primary tumors and n = 18 metastases

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 1. Immunohistochemical analyses of intratumoral and peritumoral lymphocytes. Immunohistochemical staining of tumor-infiltrating (A) Foxp3 + T cells and (B) CD8 + T lymphocytes in invasive breast cancer. A diffuse pattern of intratumoral lymphocytes was observed. By contrast, peritumoral lymphocytes formed a lymphoid aggregate (x10 objective lens). There were markedly more Foxp3 + Tregs in the (C) peritumoral area than the (E) intratumoral areas. There was an increased number of CD8 + lymphocytes in the (D) peritumoral area than the (F) intratumoral areas. A and B, x10 objective lens; B-E, x40 objective lens. CD, cluster of differentiation; Foxp3, forkhead box 3.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 3 UVR-induced inflammation recruits and maintains CD8 + GATA3 + T cells 14 days post-UVR . (a) Quantification of the total number of CD8 + cells on immunohistochemical staining ( N = 12 or 13 healthy volunteers for each time point). (b) Percentage of CD8 + GATA3 + cells of total CD8 + cells by immunofluorescence analysis ( N = 8 healthy volunteers for each time point). (c) Representative immunofluorescent images of CD8 + GATA3 + cells post-UVR. Data are mean +- SEM. Experiments were performed once. Scale bars = 50 um. * P < 0.05, ** P < 0.01, *** P < 0.001.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 3 Transcriptomic analysis demonstrates considerable ITH underlying convergent immune phenotypes. a Volcano plot of differentially expressed genes comparing high vs. low immune infiltrate regions across the tumor core and margins. Vertical red lines indicate a minimum twofold change in expression value; horizontal red line indicates the adjusted p -value threshold of

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 1 Matched haematoxylin & eosin (H&E) stained sections and multiplexed immunofluorescence (mIF) images from TMA cores of 2 patients tumours stained using Opal reagents: CD3 white, CD8 pink, CD20 orange, CD68 purple, PD-1 turquoise, PD-L1 yellow, FoxP3 green and Pan-CK red. DAPI nuclear counterstain is dark blue.. Patient 1 ( A - D ) & patient 2 ( E - H ) ( A , E : H&E x 200; B , F: mIF x 200; C and G: mIF x 400; D and H: H&E x 400).

MA5-13473