MA5-18156

antibody from Invitrogen Antibodies

Targeting: KRT76 HUMCYT2A, KRT2B, KRT2P

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-18156

Provider

Invitrogen Antibodies

Product name

Cytokeratin Pan Monoclonal Antibody (C-11), Alexa Fluor™ 488

Antibody type

Monoclonal

Antigen

Other

Description

This antibody reacts with Cytokeratin peptides 4, 5, 6, 8, 10, 13, 18.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Green dye

Isotype

IgG

Antibody clone number

C-11

Vial size

100 µg

Concentration

0.1 mg/mL

Storage

4° C, store in dark, DO NOT FREEZE!

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Cytokeratin Pan was performed using 70% confluent log phase A-431 cells. The cells were fixed and permeabilized with ice-cold acetone and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin Pan Monoclonal Antibody (C11), Alexa Fluor 488 (Product # MA5-18156) at 1:100 dilution in 0.1% BSA and incubated at 4 degree Celsius overnight (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing cytoskeletal localization. Panel e represents the isotype control. The images were captured at 60X magnification.

Conjugate

Green dye

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis (intracellular staining) of cytokeratin expression in HeLa cells and in peripheral blood cells using anti-cytokeratin antibody (C-11) Alexa Fluor® 488 Monoclonal antibody (Product # MA5-18156).

Conjugate

Green dye