Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 22-0425-71 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD4/CD25 Antibody Cocktail, APC, FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This product consists of two fluorochrome-conjugated monoclonal antibodies to identify CD4+CD25+ human peripheral blood cells. The RPA-T4 monoclonal antibody (mouse IgG1, kappa) reacts with human CD4, a 59 kDa cell surface receptor that is expressed by a majority of thymocytes, a subpopulation of mature T cells (T helper cells) and at low levels on monocytes. The BC96 monoclonal antibody (mouse IgG1, kappa) reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). This cocktail of RPA-T4 and BC96 can be used for the flow cytometric detection of human regulatory T cells, which also express Foxp3. Applications Reported: This product has been reported for use in flow cytometric analysis. Applications Tested: This cocktail has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL per test (containing 1 µg of Anti-Human CD4 (RPA-T4) FITC and 0.125 µg of Anti-Human CD25 (BC96) APC). A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- RPA-T4, BC96
- Vial size
- 25 Tests
- Concentration
- 20 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue and CD4/CD25 APC/FITC cocktail. As expected based on known relative expression patterns, CD4 clone RPA-T4 and CD25 clone BC96 stains a subset of lymphocytes, respectively.