PA5-36038

antibody from Invitrogen Antibodies

Targeting: MAPK3 ERK1, p44erk1, p44mapk, PRKM3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA5-36038

Provider

Invitrogen Antibodies

Product name

Phospho-ERK1/ERK2 (Tyr204) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 42 and 44 kDa. Purity is >95% by SDS-PAGE.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-ERK1/ERK2 (Tyr204) in L02 whole cell lysate (treated with 1,000 ng/mL LPS for 15 min), untreated (lane 1) or treated with lambda-Ppase (lane 2). Samples were incubated with Phospho-ERK1/ERK2 (Tyr204) polyclonal antibody (Product # PA5-36038) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-ERK1/ERK2 (Tyr204) in Lane 1: HeLa whole cell lysate treated with UV (4h), Lane 2: NIH-3T3 whole cell lysate treated with UV (4h), Lane 3: PC12 whole cell lysate treated with UV (4h). Samples were incubated with Phospho-ERK1/ERK2 (Tyr204) polyclonal antibody (Product # PA5-36038) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-ERK1+ERK2 pTyr204 using Phospho-ERK1+ERK2 pTyr204 polyclonal antibody (Product # PA5-36038) at a dilution of 1:500. Lane 1: Hela whole cell lysate treated with UV (4h), Lane 2: NIH-3T3 whole cell lysate treated with UV (4h), Lane 3: PC12 whole cell lysate treated with UV (4h).