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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- MA5-16308-D650 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- beta Tubulin Loading Control Monoclonal Antibody (BT7R), DyLight™ 650
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA5-16308-D650 has successfully been used for immunofluorescence. DyLight 650 has an excitation/emission of 652/672nm.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Rabbit
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Antibody clone number
- BT7R
- Vial size
- 50 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of Beta-Tubulin (red) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a DyLight 650-conjugated Beta-Tubulin monoclonal antibody (Product # MA5-16308-D650) at a dilution of 1:50 for at least 1 hour at room temperature. F-actin (green) was stained with DyLight 488 Phalloidin (Product # 21833) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
