MA5-16308-D680

antibody from Invitrogen Antibodies

Targeting: TUBB6 HsT1601, MGC4083

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-16308-D680

Provider

Invitrogen Antibodies

Product name

beta Tubulin Loading Control Monoclonal Antibody (BT7R), DyLight™ 680

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA5-16308-D680 has successfully been used in immunofluorescence and Western blot applications. DyLight 680 has an excitation/emission of 692/712 nm.

Reactivity

Human, Mouse, Rat, Canine, Chicken/Avian, Rabbit

Host

Mouse

Conjugate

Near infrared dye

Isotype

IgG

Antibody clone number

BT7R

Vial size

50 µL

Concentration

1 mg/mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Beta-Tubulin was performed by loading 75 µg of various cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a DyLight 680-conjugated Beta-Tubulin monoclonal antibody (Product # MA5-16308-D680) at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using the LI-COR Odyssey.

Conjugate

Near infrared dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of COS-7 (Lane 1), MDCK (Lane 2), C2C12 (Lane 3), MDA-MB-231 (Lane 4), PC-12 (Lane 5), RSC96 (Lane 6), tissue extracts of Mouse Lung (Lane 7) and Rat Stomach (Lane 8). The blot was probed with beta Tubulin Loading Control Monoclonal Antibody (BT7R), DyLight 680 (Product # MA5-16308-D680, 1 µg/mL). A 50 kDa band corresponding to beta Tubulin was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary antibody following blocking with 5 % skimmed milk. Fluorescent detection was performed using the Odyssey® Fc imaging system (Li-cor Biosciences).

Conjugate

Near infrared dye

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Beta-Tubulin (red) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a DyLight 680-conjugated Beta-Tubulin monoclonal antibody (Product # MA5-16308-D680) at a dilution of 1:50 for at least 1 hour at room temperature and washed with PBS. F-actin (green) was stained with DyLight 488 Phalloidin (Product # 21833) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.

Conjugate

Near infrared dye