- MA5-16308-HRP - Invitrogen Antibodies TUBB6 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of Beta-Tubulin was performed by loading 50 µg of various cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated Beta-Tubulin monoclonal antibody (Product # MA5-16308-HRP) at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
Conjugate: Horseradish Peroxidase

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), C2C12 (Lane 4), MDA-MB-231 (Lane 5), PC-12 (Lane 6), RSC96 (Lane 7) and tissue extracts of Mouse Lung (Lane 8). The blot was probed with beta Tubulin Loading Control Monoclonal Antibody (BT7R), HRP (Product # MA5-16308-HRP, 1 µg/mL) and detected by chemiluminescence. A 50 kDa band corresponding to beta Tubulin was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Conjugate: Horseradish Peroxidase

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), C2C12 (Lane 4), MDA-MB-231 (Lane 5), PC-12 (Lane 6), RSC96 (Lane 7) and tissue extracts of Mouse Lung (Lane 8). The blot was probed with beta Tubulin Loading Control Monoclonal Antibody (BT7R), HRP (Product # MA5-16308-HRP, 1 µg/mL) and detected by chemiluminescence. A 50 kDa band corresponding to beta Tubulin was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10% Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Conjugate: Horseradish Peroxidase

Supportive validation

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 7 tappAS analysis results and experimental validation AltTEM processing of Ctnnd1 /p120 . a Protein-level visualization of tappAS functional annotation for Ctnnd/p120 . Exclusion of an exon in two of its isoforms causes an NLS motif to appear in the protein sequence. b Gene-, transcript-, and CDS-level expression of Ctnnd1 . The gene is significant for both DIU and DCU, with major isoform switching of the nuclear isoforms (yellow and red) in OPCs. c DFI analysis results for the NLS motif in Ctnnd1 . NLS inclusion is favored in OPCs. d Western blot analysis of Ctnnd1 protein p120 in nuclear cytosolic, cytosolic, and total fractions of NPCs and OPCs. An increase of the nuclear expression of the protein is observed in OPCs due to differential inclusion of the NLS, while cytosolic expression remains constant
Conjugate: Horseradish Peroxidase

MA5-16308-HRP