Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- MA5-16308-A488 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- beta Tubulin Loading Control Monoclonal Antibody (BT7R), Alexa Fluor™ 488
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Rabbit
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- BT7R
- Vial size
- 50 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of beta-Tubulin was performed by loading various cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a low fluorescence PVDF membrane and blocked with Sea Block blocking buffer for at least 1 hour. The membrane was probed with a AlexaFluor488-conjugated beta-Tubulin monoclonal antibody (Product # MA5-16308-A488) at a dilution of 1:500 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using a fluorescence imaging system.
- Conjugate
- Green dye