Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
- Other assay [5]
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- Product number
- MA5-11732 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- beta Tubulin Monoclonal Antibody (TBN06 (Tub 2.5))
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- TBN06 (Tub 2.5)
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references FoxM1 drives ADAM17/EGFR activation loop to promote mesenchymal transition in glioblastoma.
Zhang C, Han X, Xu X, Zhou Z, Chen X, Tang Y, Cheng J, Moazzam NF, Liu F, Xu J, Peng W, Du F, Zhang B, Song Z, Zeng J, Gong A
Cell death & disease 2018 May 1;9(5):469
Cell death & disease 2018 May 1;9(5):469
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Supportive validation
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- Western blot analysis of Beta Tubulin was performed by loading 20 µg of the indicated whole cell lysates and 5 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BX10). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. Beta-Tubulin was detected at ~55 kDa using a Beta Tubulin mouse monoclonal antibody (Product # MA5-11732) at a concentration of 1 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177) at a dilution of 1:1000 for at least one hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078).
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot of Tubulin Beta using Tubulin Beta Monoclonal Antibody (Product # MA5-11732) on LS174T Cells.
Supportive validation
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- Experimental details
- Immunofluorescent analysis of beta-tubulin (green) in HeLa cells. Cells were fixed and permeabilized with ice-cold methanol for 10 minutes at room temperature, and blocked with 0.3% BSA in PBS for at least 15 minutes at room temperature. Cells were probed with a beta-tubulin monoclonal antibody (Product # MA5-11732) at a dilution of 1:100 (right panel), or incubated in blocking buffer as a negative control (left panel) overnight at 4°C. Cells were washed with PBS, and incubated with a DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for at least 1 hour at room temperature. Nuclei (blue) were stained with DAPI (Product # 46190). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
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- Immunofluorescent analysis of beta-tubulin (green) in COS7 and NRK cells. Cells were fixed and permeabilized with ice-cold methanol for 10 minutes at room temperature, and blocked with 0.3% BSA in PBS for at least 15 minutes at room temperature. Cells were probed with a beta-tubulin monoclonal antibody (Product # MA5-11732) at a dilution of 1:50 overnight at 4°C. Cells were washed with PBS, and incubated with a DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for at least 1 hour at room temperature. Nuclei (blue) were stained with DAPI (Product # 46190). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
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- Immunofluorescent analysis of Beta- Tubulin (green) in HeLa cells untreated or treated with 1uM Actinomycin D for 19 hours. The cells were fixed with 4% Paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a Beta-Tubulin mouse monoclonal antibody (Product # MA5-11732) at a concentration of 5 µg/mL in blocking buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32723) at a dilution of 1:500 for at least 30 minutes at a room temperature in the dark (green). Nuclei (blue) were stained with Hoechst 33342 (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
Supportive validation
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- Formalin-fixed, paraffin-embedded human lung stained with Tubulin beta antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic and surface staining of ciliated epithelial cells in bronchiole.
Supportive validation
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- Fig. 1 The expression profiles of FoxM1 and ADAM17 are positively correlated with mesenchymal features in GBM. a Heatmap of the gene expression profile revealed that FoxM1 and ADAM17 expression profiles were associated with mesenchymal markers expression in glioma specimens from TCGA database. b FoxM1, ADAM17 and mesenchymal markers expression in glioma cells were detected by western blot. beta-Tubulin was used as a loading control. c The transwell migration assay was conducted to count the migrated cells of four glioma cell lines. Scale bar = 100 mum. The data are shown as the mean +- s.d. of three independent experiments. Student's t test was used to determine the significance of the differences between the groups ( # P < 0.0001, Student's t test) . d The conditional medium was used to investigate the osteogenesis and adipogenesis potential of four glioma cell lines. Scale bar = 100 mum. Ost osteogenesis, Adi adipogenesis
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- Figure 10. Analysis of t-BID-dependent phosphoproteome. (a) Representation of the functional protein interaction network of the tBID phosphoproteome. Proteins, containing at least one phosphopeptide undergoing a change in phosphorylation after tBID delivery (light gray; q-value < 0.04) are represented in a STRING network (high-confidence, score 0.7). Only proteins with at least one connection in STRING are shown. Colored circles depict the biological annotation of proteins as obtained from DAVID ( Table S5 ). (b) Graphical representation of CASP3 known (color) and predicted substrates (gray) as shown in Table S1 . (c) Confocal images of HeLa cells infected with either Delta HOPEMT asd + YopE 1-138 or Delta HOPEMT asd + YopE 1-138 -tBID reveal the induction of an apoptotic phenotype after tBID delivery. Cells were stained for the nuclei with Hoechst, for F-actin with phalloidin, for tubulin with an anti-tubulin antibody, and for mitochondria with Mitotracker. Bars, 40 um.
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- Figure 2 Modulation of cell mechanosensors and proliferation. ( A , B ) Representative immunoblots of beta actin, beta tubulin, and beta1 integrin expression levels in extracts from control cells at 1g (Ctr) or cells exposed to s-microgravity (RPM) at different exposure times (24-96 h). The densitometric analyses are plotted as the relative expression calculated as a ratio between the optical density (OD) x mm 2 of each band and OD x mm 2 of the corresponding GAPDH band, used as loading control. ( C ) Cell proliferation tested on Ctr and RPM-exposed cells at different exposure times (24-96 h). The data are presented as the means +- SEM from three independent experiments. * p < 0.05 vs. Ctr .
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- Figure 2 GSS treatment inhibited microglial M1 polarization and alpha7nAChR-NF-kappaB signaling activation in tMCAO rats. Microglial M1 depolarization and alpha7nAChR-NF-kappaB signaling activation in the ischemic penumbra region was determined 24 h I/R. (A) Microglia morphology was determined by immunofluorescent staining of CD11b (Scale bar = 20 mum, n=6). (B-F) Western results of IL-1beta, CD11b, CD40 and CD68. (G-I) CD11b, CD40 and CD68 mRNA expression determined by qPCR. (J) Representative images of immunofluorescent staining of alpha7nAChR (Scale bar = 20 mum, n=6). (K-O) Western blot results of alpha7nAChR and NF-kappaB signaling proteins. (P-R), the qPCR results of IKK, IkappaB and P65-NF-kappaB mRNA expression. Comparisons between groups were carried out using one-way ANOVA followed by Newman-Keuls test. *P
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- FIGURE 3 Meiotic progression of BRD2 and NPM1-inhibited oocytes following 6 h of in vitro maturation (IVM). (A) Representative images of meiotic stages: GV- germinal vesicle, GVBD- germinal vesicle break-down, MI- metaphase I, and MII- metaphase II. Top images: merged FITC (anti-beta-Tubulin) and DAPI images. Outer dashed line delineates oocyte boundary. Scale bar = 50 mum. Lower images in squares: magnified area of chromatin configuration (DAPI) and meiotic spindle (beta-Tubulin). Scale bar = 10 mum. cc = cumulus cells. (B,D) Bar graphs showing the percentage of oocytes in each meiotic stage following protein inhibition and 6 h of IVM. GV, GVBD, MI, MII, Parth.- parthenotes, Deg.- degraded. (C,E) Bar graphs showing percentage of oocytes that entered meiosis following 6 h of IVM. Red bars = indicated protein inhibited, Gray bars = mock transfection control. Bars show mean +- SEM (3 replicates). * P < 0.05.