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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-62085 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DIO1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PHPSQFETAR GPHPNQFEGP RGQAPNFMPG PRGIQPQQFE DQRVHSPPRF TNQRAPAPLQ FGGLRGSAPF SEKNEQTPSR FHFQGQ Highest antigen sequence identity to the following orthologs: Mouse - 79%, Rat - 79%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DIO1 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4. Samples were probed using a DIO1 Polyclonal Antibody (Product # PA5-62085).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DIO1 in human cell line U-2 OS shows positivity in vesicles & nucleus but excluded from the nucleoli. Samples were probed using a DIO1 Polyclonal Antibody (Product # PA5-62085).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DIO1 in human rectal tissue shows strong nuclear positivity in glandular cells. Samples were probed using a DIO1 Polyclonal Antibody (Product # PA5-62085).
