Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [2]
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Validation data
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- Product number
- AF660 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CTGF/CCN2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CTGF/CCN2 C-Terminus in direct ELISAs. In direct ELISAs, approximately 15% cross-reactivity with recombinant rat CTGF is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
SPARC mediates early extracellular matrix remodeling following myocardial infarction.
Connective tissue growth factor and its correlation to other growth factors in experimental granulation tissue.
Riordan DP, Varma S, West RB, Brown PO
PloS one 2015;10(7):e0128975
PloS one 2015;10(7):e0128975
SPARC mediates early extracellular matrix remodeling following myocardial infarction.
McCurdy SM, Dai Q, Zhang J, Zamilpa R, Ramirez TA, Dayah T, Nguyen N, Jin YF, Bradshaw AD, Lindsey ML
American journal of physiology. Heart and circulatory physiology 2011 Aug;301(2):H497-505
American journal of physiology. Heart and circulatory physiology 2011 Aug;301(2):H497-505
Connective tissue growth factor and its correlation to other growth factors in experimental granulation tissue.
Inkinen K, Wolff H, Lindroos P, Ahonen J
Connective tissue research 2003;44(1):19-29
Connective tissue research 2003;44(1):19-29
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- CTGF/CCN2 in Human Kidney. CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human kidney array using Goat Anti-Human CTGF/CCN2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- CTGF/CCN2 in Human Pancreas. CTGF/CCN2 was detected in immersion fixed paraffin-embedded sections of human pancreas array using Goat Anti-Human CTGF/CCN2 C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF660) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.