Antibody data
- Antibody Data
- Antigen structure
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- Product number
- TAB1001 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GAPDH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- TAB1001 detects GAPDH in samples.
- Concentration
- 1 mg/mL
Submitted references A Helicase Unwinds Hexanucleotide Repeat RNA G-Quadruplexes and Facilitates Repeat-Associated Non-AUG Translation.
Implications of cell division cycle associated 4 on the Wilm's tumor cells viability via AKT/mTOR signaling pathway.
Tetraspanin CD82 is necessary for muscle stem cell activation and supports dystrophic muscle function.
G-quadruplexes offer a conserved structural motif for NONO recruitment to NEAT1 architectural lncRNA.
Targeting viperin to the mitochondrion inhibits the thiolase activity of the trifunctional enzyme complex.
Molecular determinants of SR-B1-dependent Plasmodium sporozoite entry into hepatocytes.
miR‑378a‑3p inhibits cellular proliferation and migration in glioblastoma multiforme by targeting tetraspanin 17.
Tumor Necrosis Factor-α-Mediated Metaplastic Inhibition of LTP Is Constitutively Engaged in an Alzheimer's Disease Model.
L3MBTL1 regulates ALS/FTD-associated proteotoxicity and quality control.
A novel null mutation in the pyruvate dehydrogenase phosphatase catalytic subunit gene (PDP1) causing pyruvate dehydrogenase complex deficiency.
Viperin interacts with the kinase IRAK1 and the E3 ubiquitin ligase TRAF6, coupling innate immune signaling to antiviral ribonucleotide synthesis.
Sumoylation promotes optimal APC/C Activation and Timely Anaphase.
BET bromodomain inhibitors and agonists of the beta-2 adrenergic receptor identified in screens for compounds that inhibit DUX4 expression in FSHD muscle cells.
HSP90 stabilizes B-cell receptor kinases in a multi-client interactome: PU-H71 induces CLL apoptosis in a cytoprotective microenvironment.
Rapamycin rescues vascular, metabolic and learning deficits in apolipoprotein E4 transgenic mice with pre-symptomatic Alzheimer's disease.
MeCP2 Affects Skeletal Muscle Growth and Morphology through Non Cell-Autonomous Mechanisms.
Activated Met signalling in the developing mouse heart leads to cardiac disease.
Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation.
Long-range transcriptional control of progesterone receptor gene expression.
Apurinic/apyrimidinic endonuclease 1 alters estrogen receptor activity and estrogen-responsive gene expression.
Thioredoxin and thioredoxin reductase influence estrogen receptor alpha-mediated gene expression in human breast cancer cells.
Liu H, Lu YN, Paul T, Periz G, Banco MT, Ferré-D'Amaré AR, Rothstein JD, Hayes LR, Myong S, Wang J
Journal of the American Chemical Society 2021 May 19;143(19):7368-7379
Journal of the American Chemical Society 2021 May 19;143(19):7368-7379
Implications of cell division cycle associated 4 on the Wilm's tumor cells viability via AKT/mTOR signaling pathway.
Li S, Qin C, Chen Y, Wei D, Tan Z, Meng J
Renal failure 2021 Dec;43(1):1470-1478
Renal failure 2021 Dec;43(1):1470-1478
Tetraspanin CD82 is necessary for muscle stem cell activation and supports dystrophic muscle function.
Hall A, Fontelonga T, Wright A, Bugda Gwilt K, Widrick J, Pasut A, Villa F, Miranti CK, Gibbs D, Jiang E, Meng H, Lawlor MW, Gussoni E
Skeletal muscle 2020 Nov 27;10(1):34
Skeletal muscle 2020 Nov 27;10(1):34
G-quadruplexes offer a conserved structural motif for NONO recruitment to NEAT1 architectural lncRNA.
Simko EAJ, Liu H, Zhang T, Velasquez A, Teli S, Haeusler AR, Wang J
Nucleic acids research 2020 Jul 27;48(13):7421-7438
Nucleic acids research 2020 Jul 27;48(13):7421-7438
Targeting viperin to the mitochondrion inhibits the thiolase activity of the trifunctional enzyme complex.
Dumbrepatil AB, Zegalia KA, Sajja K, Kennedy RT, Marsh ENG
The Journal of biological chemistry 2020 Feb 28;295(9):2839-2849
The Journal of biological chemistry 2020 Feb 28;295(9):2839-2849
Molecular determinants of SR-B1-dependent Plasmodium sporozoite entry into hepatocytes.
Langlois AC, Manzoni G, Vincensini L, Coppée R, Marinach C, Guérin M, Huby T, Carrière V, Cosset FL, Dreux M, Rubinstein E, Silvie O
Scientific reports 2020 Aug 11;10(1):13509
Scientific reports 2020 Aug 11;10(1):13509
miR‑378a‑3p inhibits cellular proliferation and migration in glioblastoma multiforme by targeting tetraspanin 17.
Guo XB, Zhang XC, Chen P, Ma LM, Shen ZQ
Oncology reports 2019 Nov;42(5):1957-1971
Oncology reports 2019 Nov;42(5):1957-1971
Tumor Necrosis Factor-α-Mediated Metaplastic Inhibition of LTP Is Constitutively Engaged in an Alzheimer's Disease Model.
Singh A, Jones OD, Mockett BG, Ohline SM, Abraham WC
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Nov 13;39(46):9083-9097
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Nov 13;39(46):9083-9097
L3MBTL1 regulates ALS/FTD-associated proteotoxicity and quality control.
Lu J, Periz G, Lu YN, Tang Q, Liu Y, Zhang T, Shah Y, Thombre R, Aljumaah R, Li W, Mojsilovic-Petrovic J, Ji Y, Johnson K, Kalb R, Wang J
Nature neuroscience 2019 Jun;22(6):875-886
Nature neuroscience 2019 Jun;22(6):875-886
A novel null mutation in the pyruvate dehydrogenase phosphatase catalytic subunit gene (PDP1) causing pyruvate dehydrogenase complex deficiency.
Bedoyan JK, Hecht L, Zhang S, Tarrant S, Bergin A, Demirbas D, Yang E, Shin HK, Grahame GJ, DeBrosse SD, Hoppel CL, Kerr DS, Berry GT
JIMD reports 2019 Jul;48(1):26-35
JIMD reports 2019 Jul;48(1):26-35
Viperin interacts with the kinase IRAK1 and the E3 ubiquitin ligase TRAF6, coupling innate immune signaling to antiviral ribonucleotide synthesis.
Dumbrepatil AB, Ghosh S, Zegalia KA, Malec PA, Hoff JD, Kennedy RT, Marsh ENG
The Journal of biological chemistry 2019 Apr 26;294(17):6888-6898
The Journal of biological chemistry 2019 Apr 26;294(17):6888-6898
Sumoylation promotes optimal APC/C Activation and Timely Anaphase.
Lee CC, Li B, Yu H, Matunis MJ
eLife 2018 Mar 8;7
eLife 2018 Mar 8;7
BET bromodomain inhibitors and agonists of the beta-2 adrenergic receptor identified in screens for compounds that inhibit DUX4 expression in FSHD muscle cells.
Campbell AE, Oliva J, Yates MP, Zhong JW, Shadle SC, Snider L, Singh N, Tai S, Hiramuki Y, Tawil R, van der Maarel SM, Tapscott SJ, Sverdrup FM
Skeletal muscle 2017 Sep 4;7(1):16
Skeletal muscle 2017 Sep 4;7(1):16
HSP90 stabilizes B-cell receptor kinases in a multi-client interactome: PU-H71 induces CLL apoptosis in a cytoprotective microenvironment.
Guo A, Lu P, Lee J, Zhen C, Chiosis G, Wang YL
Oncogene 2017 Jun 15;36(24):3441-3449
Oncogene 2017 Jun 15;36(24):3441-3449
Rapamycin rescues vascular, metabolic and learning deficits in apolipoprotein E4 transgenic mice with pre-symptomatic Alzheimer's disease.
Lin AL, Jahrling JB, Zhang W, DeRosa N, Bakshi V, Romero P, Galvan V, Richardson A
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2017 Jan;37(1):217-226
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2017 Jan;37(1):217-226
MeCP2 Affects Skeletal Muscle Growth and Morphology through Non Cell-Autonomous Mechanisms.
Conti V, Gandaglia A, Galli F, Tirone M, Bellini E, Campana L, Kilstrup-Nielsen C, Rovere-Querini P, Brunelli S, Landsberger N
PloS one 2015;10(6):e0130183
PloS one 2015;10(6):e0130183
Activated Met signalling in the developing mouse heart leads to cardiac disease.
Leo C, Sala V, Morello M, Chiribiri A, Riess I, Mancardi D, Schiaffino S, Ponzetto C, Crepaldi T
PloS one 2011 Feb 9;6(2):e14675
PloS one 2011 Feb 9;6(2):e14675
Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation.
Kuo JC, Han X, Hsiao CT, Yates JR 3rd, Waterman CM
Nature cell biology 2011 Apr;13(4):383-93
Nature cell biology 2011 Apr;13(4):383-93
Long-range transcriptional control of progesterone receptor gene expression.
Bonéy-Montoya J, Ziegler YS, Curtis CD, Montoya JA, Nardulli AM
Molecular endocrinology (Baltimore, Md.) 2010 Feb;24(2):346-58
Molecular endocrinology (Baltimore, Md.) 2010 Feb;24(2):346-58
Apurinic/apyrimidinic endonuclease 1 alters estrogen receptor activity and estrogen-responsive gene expression.
Curtis CD, Thorngren DL, Ziegler YS, Sarkeshik A, Yates JR, Nardulli AM
Molecular endocrinology (Baltimore, Md.) 2009 Sep;23(9):1346-59
Molecular endocrinology (Baltimore, Md.) 2009 Sep;23(9):1346-59
Thioredoxin and thioredoxin reductase influence estrogen receptor alpha-mediated gene expression in human breast cancer cells.
Rao AK, Ziegler YS, McLeod IX, Yates JR, Nardulli AM
Journal of molecular endocrinology 2009 Dec;43(6):251-61
Journal of molecular endocrinology 2009 Dec;43(6):251-61
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Supportive validation
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- Western blot analysis of GAPDH using a polyclonal antibody (Product # TAB1001).
Supportive validation
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- Figure 2 Analysis of Met expression and downstream signalling activation in neonatal cardiomyocytes. (A) Immunofluorescence of Met receptor (red) and GFP (green) in neonatal (P7) heart samples of control (left panel) and HGF tg mice (middle panel). A negative control of secondary antibody was included (right panel). Bars: 50um. (B) Western blot of Met (p140Met) protein in control and HGF tg mice at different ages post-birth (P2 n = 6 n = 7, P4 n = 8 n = 6, P7 n = 10 n = 11, P18 n = 9 n = 14). Representative blots are shown below densitometric quantification (normalized on GAPDH loading control, relative to P2 control). Controls vs HGF tg mice: *p
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- Fig 4 Muscle specific deletion of Mecp2 does not lead to the observed muscular alterations. (A) WBs confirm the specific skeletal muscle deletion of Mecp2 in the transgenic Mecp2 Flox MyoD iCre mice. WT, Mecp2 Flox and MyoD iCre genotypes are used as controls. (B) Representative TA cross-sections stained with Hematoxylin and Eosin (upper panels) or immunostained for Laminin (bottom panels). Scale bar = 50 mum. (C) Mean CSA +- s.e.m. showing no statistically significant differences between Mecp2 Flox MyoD iCre mice fibers and control ones. At least 130 myofibers were counted for each animal. Significance was calculated using one-way ANOVA (ns: 0.8520). (D) Mecp2 Flox MyoD iCre mice and controls show similar levels of Gast IGF-1 mRNA. Significance was calculated using one-way ANOVA (n>=3; ns: 0.9283). (E) Graph showing similar levels of P-rpS6 between Mecp2 Flox MyoD iCre quadriceps and the corresponding controls. Data are represented as mean +- s.e.m. (n = 2).
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- Fig 2 Mecp2 -/y muscles exhibit deregulated protein synthesis signalling pathways. (A) qPCR evaluation of IGF-1 (n = 8) and IGF-R (n = 3) mRNA expression in WT and Mecp2 -/y gastrocnemius. All data points were calculated in triplicate, normalized to GAPDH and represented as gene expression relative to WT expression. Significance is calculated with t test (**** P value:
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- Figure 6. NONO binds to NEAT1 through G-quadruplex motifs. ( A ) Quadruplex-forming G-rich sequences (QGRS) mapped to NEAT1 are shown as tall bars (black). ( B ) NONO eCLIP data mapped to NEAT1. Reads are plotted by intensity and called peaks are shown as short bars. Tracks, from top to bottom: Merged peaks, determined by replication and adjustment for input (dark purple); Replicate 1 read intensity plot with called peaks directly beneath (purple); Replicate 2 read intensity plot with called peaks directly beneath (pink); size-matched paired input read intensity plot (grey). The statistical significance of overlap between QGRS and each set of peaks was determined using the two-tailed Fisher's exact test, with P -values shown in parentheses beneath each corresponding track label. ( C ) Circular dichroism of 5 muM NEAT1_425 and NEAT1_22619 G-quadruplex RNAs following addition of TMPyP4 at increasing concentrations. ( D ) NEAT1 enrichment by NONO RNA-IP with addition of TMPyP4. RNA-IP for NONO was performed on HEK293T cell lysate. Various concentrations of TMPyP4 were added to lysate before immunoprecipitation. RT-qPCR was performed to measure NEAT1 enrichment, expressed as percentage of input ( n = 4). '***' indicates P < 0.0005; 'n.s.' indicates P >= 0.05 (two-tailed t -test). ( E ) Western blot of RNA-IP samples described in (D) and 5% input. GAPDH was blotted as a negative control for IP enrichment.
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- Figure 1. APC4 is sumoylated in a cell-cycle-dependent manner at two C-terminal lysines. ( A ) HeLa cells were synchronized in S-phase using a double-thymidine arrest and released for varying time points. Whole cell lysates were analyzed by immunoblotting for APC4, Cyclin B1, Cdc20, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a loading control. Asterisks indicate sumoylated forms of APC4. ( B ) APC4 contains two C-terminal SUMO consensus site lysines at 772 and 798. ( C ) Full-length wild-type APC4 or the indicated lysine to alanine substitution mutants were expressed in rabbit reticulocyte lysate in the presence of [ 35 S]-methionine and incubated for the indicated times in modification reactions containing SUMO E1 and E2 enzymes and SUMO2. Proteins were detected by SDS-PAGE and autoradiography. Asterisks indicate sumoylated forms of APC4. ( D ) Constructs coding for FLAG-tagged versions of wild type APC4 or a sumoylation-deficient mutant containing arginine substitutions at lysines 772 and 798 (APC4 KR ) were used to generate stable inducible cell lines in YFP-H2B HeLa cells. ( E ) Endogenous APC4 was depleted by siRNA, and FLAG-APC4 or FLAG-APC4 KR stable cell lines were induced by doxycycline for 48 hr. Immunoblot analysis using APC4 and tubulin-specific antibodies reveals that FLAG-APC4 and FLAG-APC4 KR are expressed at near endogenous levels. ( F-G ) Co-immunoprecipitations were performed with an antibody against APC4, followed by immunoblotting for APC4
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- 10.7554/eLife.29539.007 Figure 2. APC4 sumoylation is required for timely metaphase-anaphase transition. ( A ) Cells were transfected with control or APC4-specific siRNAs for 48 hr followed by 16 hr of timelapse live cell acquisition. siAPC4 transfected cells were also induced to express FLAG-APC4 or FLAG-APC4 KR . Analysis represents mitotic progression time beginning with nuclear envelope breakdown (NEBD) to anaphase onset. Quantification of mitotic phenotypes is shown. Prolonged metaphase is defined by >60 min in metaphase plate alignment before anaphase onset. Abnormal metaphase is defined by inability to generate a metaphase plate and defects in chromosomal cohesion. n > 100 for each cell line. ( B ) Cells representative of each mitotic phenotype categorized in ( A ) are featured with timestamps in minutes. ( C ) Mitotic progression beginning with NEBD to metaphase plate alignment and from metaphase plate alignment to anaphase onset was quantified in FLAG-APC4 and FLAG-APC4 KR expressing cells. Experiments were performed in triplicate; means are displayed and error bars represent standard deviations. n = 50 for each cell line. Two-tailed t-tests were used to calculate significance: p=0.38 for differences in FLAG-APC4 and FLAG-APC4 KR timing from NEBD-metaphase, p
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- Figure 3 Mouse SR-B1 poorly supports P. berghei sporozoite invasion. (A,B) CD81KOH16 cells were transfected with either mouse or human SR-B1 plasmids, or no plasmid as a control (Mock). Total protein expression was analyzed using polyclonal anti-SR-B1 antibodies (Ab24603) by western blot (A) with GAPDH as a loading control. Surface protein expression was analyzed by flow cytometry (B) using anti-human ""alphaH"" SR-B1 polyclonal rabbit serum (blue) and anti-mouse ""alphaM"" polyclonal antibodies NB400-113 (orange). The grey histogram represents untransfected cells labeled with the corresponding antibody. (C,D) CD81KOH16 (C) and WT Hepa1-6 cells treated with siRNA against CD81 24 h before (D) , were transfected with mouse or human SR-B1 plasmids, or no plasmid as a negative control (Mock), and then infected with PbGFP sporozoites. EEF numbers were counted by microscopy after UIS4 staining, 24 h after sporozoite addition. The mean control values for each experiment were 59, 139, 214, 245, 299, 315 and 383 EEFs/well in hSR-B1-transfected CD81KOH16 cells (C) , and 30, 140, 155 and 215 EEFs/well in control Hepa1-6 cells (D) . * p < 0.05; ** p < 0.01 (repeated measures one-way ANOVA followed by Tukey's multiple comparisons test).
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- Figure 2. The influence of CDCA4-knockdown and -overexpression on the CDCA4 expression and WiT49 cells proliferation. (a-b) mRNA expression (a) and protein expression levels (b) of CDCA4 were measured by qRT-PCR (a) and western blot (b) analyses after transfected with control, si-NC, si-CDCA4-1, and si-CDCA4-2 in WiT49 cells. (c) Quantification of b. ** p < 0.01 vs. control or si-NC group. (d-e) mRNA expression (d) and protein expression levels (e) of CDCA4 were examined by qRT-PCR (d) and western blot (e) analyses after transfected with control, pcDNA3.1-vector, and pcDNA3.1-CDCA4 in WiT49 cells. (f) Quantification of e. ** p < 0.01 vs . control or vector group. (g-h) The influences of CDCA4-knockdown (g) and -overexpression (h) on the WiT49 cells proliferation were determined by CCK8 assay. ** p < 0.01 vs . si-NC or vector group.
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- Figure 4. CDCA4 affected cell cycle and AKT/mTOR signaling pathway. (a-b) GSEA was utilized to identify the relative signaling pathway associated with high CDCA4 expression. (c-f) The effects of CDCA4-knockdown (c-d) and -overexpression (e-f) on the AKT/mTOR relative markers and Cyclin D1 protein were evaluated using western blot analysis. (d) Quantification of c. (f) Quantification of e. ** p < 0.01 vs . si-NC or vector group.
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- Figure 5. Rapamycin restoring CypA levels in brain vasculature of APOE4 mice. (a,b) Immunoblots of cortical CypA lysates and the corresponding quantitative analyses; (c,d) immunoblots of microvascular CypA lysates and the corresponding quantitative analyses; (e,f) immunoblots of cortical NF-kappaB lysates and the corresponding quantitative analyses; (g,h) immunoblots of microvascular NF-kappaB lysates and the corresponding quantitative analyses. Data are presented as mean +- standard error of the mean. * P < 0.05. CypA: cyclophilin A; NF-kappaB: nuclear factor-kappab.
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- Figure 5 L3MBT1 antagonist UNC669 protects against proteotoxicity in mammaliancells. ( a ) Co-immunoprecipitation analysis of Flag-tagged L3MBTL1and endogenous p53 in HEK293 cells treated with 12.5 uM UNC669 or vehiclecontrols (n=3 independent experiments; *** P(VEH)=0.0002, *** P(UNC669)=0.0004).( b ) HEK293 cells treated with increased concentrations ofUNC669 show increased p53 protein transcriptional activity, as measured by thep53 response element (p53-RE)-mediated luciferase activity assay (n=3independent experiments; * P=0.03; ** P=0.002). ( c ) HEK293 cellsexpressing SOD1 G85R were treated with increasing concentrations ofUNC669 (top) and the S and P fractions were analyzed by western blotting andprobed with antibodies against SOD1 and GAPDH. ( d ) Quantificationof SOD1 G85R western blot analyses in (c) (n=3 independentexperiments; * P