MA1-065-A555

antibody from Invitrogen Antibodies

Targeting: CLTCL1 CHC22, CLH22, CLTCL, CLTD

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-065-A555

Provider

Invitrogen Antibodies

Product name

Clathrin Heavy Chain Monoclonal Antibody (X22), Alexa Fluor™ 555

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA1065A555 detects clathrin heavy chain in non-human primate, bovine, human, rat and mouse tissues as well as hamster (CHO) cells. The MA1065A555 immunogen is purified human brain clathrin heavy chain. Electron microscopy and proteolysis mapping demonstrate that MA1065A555 binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.

Reactivity

Human, Mouse, Rat, Bovine, Hamster

Host

Mouse

Isotype

IgG

Antibody clone number

X22

Vial size

50 µL

Concentration

1 mg/mL

Storage

4° C, store in dark, DO NOT FREEZE!

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Clathrin Heavy Chain (orange) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Clathrin Heavy Chain Monoclonal Antibody, AlexaFluor 555 conjugate (Product # MA1-065-A555) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.