Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- MA1-065-A647 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Clathrin Heavy Chain Monoclonal Antibody (X22), Alexa Fluor™ 647
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA1065A647 detects clathrin heavy chain in non-human primate, bovine, human, rat and mouse tissues as well as hamster (CHO) cells. The MA1065A647 immunogen is purified human brain clathrin heavy chain. Electron microscopy and proteolysis mapping demonstrate that MA1065A647 binding occurs towards the central hub of the triskelion, N-terminal to the light chain binding regions.
- Reactivity
- Human, Mouse, Rat, Bovine, Hamster
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Antibody clone number
- X22
- Vial size
- 50 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Clathrin Heavy Chain (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Clathrin Heavy Chain Monoclonal Antibody, AlexaFluor 647 conjugate (Product # MA1-065-A647) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
- Conjugate
- Red dye