PA5-27293

antibody from Invitrogen Antibodies

Targeting: MAPK14 CSBP1, CSBP2, CSPB1, Mxi2, p38, PRKM14, PRKM15

Antibody data

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Validation data

Product number: PA5-27293 - Provider product page
Provider: Invitrogen Antibodies
Product name: Crk Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3. Predicted reactivity: Mouse (100%), Rat (99%), Xenopus laevis (89%), Pig (98%), Chicken (91%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

MAPK14 protein structure - PA5-27293 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CRK using 30 µg of A431 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a CRK polyclonal antibody (Product # PA5-27293) at a dilution of 1:1500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Crk Polyclonal Antibody(Product # PA5-27293) and a ~34kDa band corresponding to Crk was observed across cell lines and tissues tested. Membrane enriched extracts (30ug lysate) of HeLa (Lane 1), MDA-MB-231 (Lane 2), MCF-7 (Lane 3), SH-SY5Y (Lane 4), IMR-32 (Lane 5), Caco-2 (Lane 6), NIH/3T3 (Lane 7), SK-OV-3 (Lane 8), Mouse Lung (Lane 9), Mouse Liver (Lane 10) and Mouse Brain (Lane 11), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Crk Polyclonal Antibody (Product # PA5-27293). Sample (30 µg of whole cell lysate). Lane A:NIH-3T3 12% SDS PAGE. Crk Polyclonal Antibody (Product # PA5-27293) diluted at 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Crk Polyclonal Antibody(Product # PA5-27293) and a ~34kDa band corresponding to Crk was observed across cell lines and tissues tested. Membrane enriched extracts (30ug lysate) of HeLa (Lane 1), MDA-MB-231 (Lane 2), MCF-7 (Lane 3), SH-SY5Y (Lane 4), IMR-32 (Lane 5), Caco-2 (Lane 6), NIH/3T3 (Lane 7), SK-OV-3 (Lane 8), Mouse Lung (Lane 9), Mouse Liver (Lane 10) and Mouse Brain (Lane 11), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).