GTX30969

antibody from GeneTex

Targeting: PRKN AR-JP, PARK2, parkin, PDJ

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX30969

Provider

GeneTex

Proper citation

GeneTex Cat#GTX30969, RRID:AB_10730984

Product name

Parkin antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Storage

Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

The Parkin antibody was tested to detect Parkin levels in stable Hek cells expressing Parkin. Antibody dilutions: 1:250 and 1:1000 Hek cell lysate used: 50ug (lanes 1 &4); 100 ug (lanes 2&5); 200ug (lanes 3&6).

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

The Parkin antibody was tested to pulldown enodgenously expressed Parkin in HIT-T15 cells (Lane 1, HIT-T15 cells; Lane 2, negative control) and transiently transfected Parkin in Hek cells (Lane 2, Hek cells; Lane 4 negative control). Amount of antibody used: 4ug Lysate of cells used: 1000ug