Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB1438 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Parkin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Parkin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Parkin-2 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O60260
- Isotype
- IgG
- Antibody clone number
- 323302
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Parkin by Western Blot. Western blot shows lysates of Sf 21 S. frugiperda insect ovarian cell line mock transfected or transfected with GST tagged Parkin. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Parkin Monoclonal Antibody (Catalog # MAB14381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GST tagged Parkin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.