MA5-15797

antibody from Invitrogen Antibodies

Targeting: PDK1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15797

Provider

Invitrogen Antibodies

Product name

PDK1 Monoclonal Antibody (4A11)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15797 targets PDK1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human, Non-human primate, and Rat samples. The MA5-15797 immunogen is purified recombinant fragment of human PDK1 expressed in E. Coli. MA5-15797 detects PDK1 which has a predicted molecular weight of approximately 44kDa.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

4A11

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Osteopontin counters human immunodeficiency virus type 1-induced impairment of neurite growth through mammalian target of rapamycin and beta-integrin signaling pathways.

Calvez M, Hseeh G, Benzer S, Brown AM
Journal of neurovirology 2019 Jun;25(3):384-396

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PDK1 using PDK1 monoclonal antibody (Product # MA5-15797) in NIH/3T3 (1), HeLa (2), Jurkat (3), HepG2 (4), PC-12 (5), and COS-7 (6) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using PDK1 monoclonal antibody (Product # MA5-15797) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Lovo cells using PDK1 monoclonal antibody (Product # MA5-15797) (green) and negative control (purple).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 8 AKT phosphorylation is activated via an mTORC2-mTORC1 pathway interaction, in a PDK-independent manner. Differentiated rat cortical neurons were prepared and treated as described in figure legend 4 and subjected to western analyses for the expression of protein kinase B (Akt Ser473), total Akt protein, phosphoinositide-dependent kinase-1 (PDK1 Ser421) and total PDK protein levels. a IIIB6.25-25, with/without rapamycin n = 3, p < .0001; IIIB25-100, with/without rapamycin n = 3, p < .0001. b IIIB25-100, with/without rapamycin n = 3, p = .0207