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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-15797 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDK1 Monoclonal Antibody (4A11)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15797 targets PDK1 in FACS, IF, IHC, and WB applications and shows reactivity with Human, Non-human primate, and Rat samples.
- Antibody clone number
- 4A11
- Concentration
- Conc. Not Determined
Submitted references Osteopontin counters human immunodeficiency virus type 1-induced impairment of neurite growth through mammalian target of rapamycin and beta-integrin signaling pathways.
Calvez M, Hseeh G, Benzer S, Brown AM
Journal of neurovirology 2019 Jun;25(3):384-396
Journal of neurovirology 2019 Jun;25(3):384-396
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PDK1 using PDK1 monoclonal antibody (Product # MA5-15797) in NIH/3T3 (1), HeLa (2), Jurkat (3), HepG2 (4), PC-12 (5), and COS-7 (6) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using PDK1 monoclonal antibody (Product # MA5-15797) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and brain tissues (right) using PDK1 monoclonal antibody (Product # MA5-15797) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Lovo cells using PDK1 monoclonal antibody (Product # MA5-15797) (green) and negative control (purple).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 8 AKT phosphorylation is activated via an mTORC2-mTORC1 pathway interaction, in a PDK-independent manner. Differentiated rat cortical neurons were prepared and treated as described in figure legend 4 and subjected to western analyses for the expression of protein kinase B (Akt Ser473), total Akt protein, phosphoinositide-dependent kinase-1 (PDK1 Ser421) and total PDK protein levels. a IIIB6.25-25, with/without rapamycin n = 3, p < .0001; IIIB25-100, with/without rapamycin n = 3, p < .0001. b IIIB25-100, with/without rapamycin n = 3, p = .0207
