MA3-918

antibody from Invitrogen Antibodies

Targeting: CALM3 PHKD

ELISA

Antibody data

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Validation data

Product number: MA3-918 - Provider product page
Provider: Invitrogen Antibodies
Product name: Calmodulin Monoclonal Antibody (6D4)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA3-918 detects calmodulin from bovine, chicken, Chlamydomonas, Dictyostelium, and rat samples. This antibody does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK). MA3-918 has been successfully used in Western blot, immunocytochemistry and ELISA procedures. By Western blot, this antibody detects a 17 kDa protein representing calmodulin from Dictyostelium cell lysate. Addition of EGTA to buffers completely inhibits antibody staining. Immunohistochemical staining of calmodulin in Dictyostelium cells with MA3-918 results in staining of the contractile vacuoles. The MA3-918 antigen is calmodulin purified from Dictyostelium discoideum.
Reactivity: Human, Rat, Bacteria, Bovine, Chicken/Avian
Host: Mouse
Isotype: IgG
Antibody clone number: 6D4
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

CALM3 protein structure - MA3-918 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Protein levels of sarcoplasmic reticulum Ca 2+ -handling proteins in intrinsic laryngeal muscles (ILM), cricothyroid (CT) muscle, extraocular muscles (EOM), and tibialis anterior (TA) muscle. Western blot analysis showing relative abundance of indicated proteins: calsequestrins 1 and 2 (CASQ1 and CASQ2), Sercas 1 and 2 (SERCA1 and SERCA2), calmodulin (CaM), calmodulin kinase II (CaMKII), and Orai1. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a control for protein loading, Western blot transfer and nonspecific changes in protein levels. The molecular weight, expressed in kDa, for each protein is indicated. Quantifications from three independent muscle samples, each sample containing muscles pooled from three or four different rats. Asterisks and different letter combination indicate statistical significance (* P < 0.05 and ab, ac, ad, bc, or bd P < 0.05, respectively). In ILM, CASQ 1 was more abundant than CASQ2 compared with TA. SERCA1 was less than SERCA2 in ILM and higher compared with TA. CT and the other ILM showed similar levels of the proteins studied.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Immunohistochemical fluorescence microscopy of the distributions and abundance of Symbiodiniaceae, chromatophores carbonic anhydrase, calmodulin and mucus within tagged polyp tissues of O. faveolata . ( A ) Horizontal cross-section of decalcified polyp tissues of O. faveolata collected in March 2008 of superimposed emissions from mucus (blue fluorescence), carbonic anhydrase (green fluorescence), and calmodulin (red fluorescence). ( B ) Enlargement of the box shown in A. ( C ) Horizontal cross-section of a decalcified polyp of O. faveolata collected in May 2008 of superimposed mucus (blue fluorescence), carbonic anhydrase (green fluorescence), and calmodulin (red fluorescence). Enlargement from the box shown within a full polyp shown in Supplementary Fig. S7 . Labels include: PS--primary septum; SS--secondary septum; PT--primary tentacle; ST--secondary tentacle; and OD--oral disk.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 7 Enlargement of immunohistochemical fluorescence microscopy of the distributions and abundance of carbonic anhydrase, calmodulin and mucus within tagged polyp tissues of O. faveolata . ( A ) Original enlarged image (from box 7A-B shown in Fig. 6 A) of a horizontal cross-section of decalcified polyp tissues of O. faveolata collected in March 2008 exhibiting superimposed emissions from mucus (blue fluorescence), carbonic anhydrase (green fluorescence), and calmodulin (red fluorescence). ( B ) Line tracing of A indicating individual Symbiodiniaceae. ( C ) A second enlargement (from area shown in Supplementary Figs. S5 , S6 ) image of a horizontal cross-section of decalcified polyp tissues of O. faveolata collected in May 2008 exhibiting superimposed emissions from mucus (blue fluorescence), carbonic anhydrase (green fluorescence), and calmodulin (red fluorescence). ( D ) Line tracing of C indicating individual Symbiodiniaceae are found in both the oral endoderm and the calicoblastic epithelium. Labels include: End--endoderm; Mes--mesoglea; Ect--ectoderm; Muc--mucus; PS--primary septum; SW--seawater at the time the polyps were collected; and Sk--skeleton. Tissue structure terminology follows nomenclature in Allemand et al and Peters .