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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA1-28037 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Myosin Skeletal Muscle Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Recommended positive controls: skeletal muscle. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 75 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Skeletal Muscle (Lane 1), Mouse Heart (Lane 2), Rat Skeletal Muscle (Lane 3), Rat heart (Lane 4), Mouse Brain (Lane 5), Rat Brain (Lane 6) and Mouse Liver (Lane 7). The blot was probed with Anti-Myosin Skeletal Muscle Polyclonal Antibody (Product # PA1-28037, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 220kDa band corresponding to Myosin Skeletal Muscle was observed across all tissues tested except for Mouse Brain, Rat Brain and Mouse Liver. Two non specific bands were also observed at 55 and 65kDa each.
