Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MA5-14702 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- hCG Monoclonal Antibody (34P2C2)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- The observed molecular weight of alpha-hCG is approximately 20-22 kD. The molecular weight is higher than the predicted molecular weight due to post translational modification. Product MA514702 is a smaller package size of MIH9822 (formerly sold as a Seradyn product).
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 34P2C2
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of hCG was performed by loading the indicated amounts of purified hCG protein extracted from human urine, and 15 µL of PageRuler Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane (Product # 88518) using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk for at least 1 hour at room temperature. hCG was detected at ~22 kD using a anti hCG antibody (Product # MA5-14702) at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody (Product # 31439) at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34076).