MIH9805

antibody from Invitrogen Antibodies

Targeting: CGB8

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MIH9805

Provider

Invitrogen Antibodies

Product name

hCG Monoclonal Antibody (3015)

Antibody type

Monoclonal

Antigen

Other

Description

The observed molecular weight of alpha-hCG is approximately 20-22 kD. The molecular weight is higher than the predicted molecular weight due to post translational modification.

Antibody clone number

3015

Concentration

5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of hCG was performed by loading the indicated amounts of protein purified hCG protein extracted from human urine, and 15 µL of PageRuler Prestained Protein Ladder (Product # 26619) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane (Product # 88518) using the G2 Fast Blotter (Product # 62288) and blocked with 5% Milk for at least 1 hour at room temperature. hCG was detected at ~22 kD using an anti hCG antibody (Product # MIH9805) at a concentration of 4 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG (Fc) secondary antibody (Product # 31439) at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34076).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of hCG was performed on human placenta tissue and is showing positive staining in extra cellular spaces. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with hCG monoclonal antibody (Product # MIH9805) at a dilution of 1:20, overnight at 4C in a humidified chamber without (left panel) or with hCG antibody control (right panel). Tissues were washed, and incubated with secondary antibody (conjugated with HRP) for 30 min at room temperature in a humidified chamber. Detection was performed using DAB substrate kit. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting. Images were taken at 20x magnification.