Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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Validation data
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- Product number
- 14-6077-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ub-K63 Monoclonal Antibody (HWA4C4), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: Ubiquitin is a highly conserved 76kDa protein which is ligated to many proteins in both monomeric and polymeric forms. Ubiquitin polymers (polyubiquitin) can be found in several varieties depending on which of its 7 lysine residues is the site of ligation. The two most common polymer chains are linked via an isopeptide bond between glycine-76 and either lysine-48 (K-48) or lysine-63 (K-63). K-48-linked polyubiquitination typically results in targeting the protein to the 26S proteosome resulting in proteolysis, although recently exceptions to this have been observed. In contrast, K-63-linked polyubiquitination results in signals related to intracellular trafficking, cell signaling, ribosomal biogenesis, and DNA damage repair. There are also reports that K63-linked polyubiquitin is involved in proteosome-independent proteolysis via autophagy.
- Antibody clone number
- HWA4C4
- Concentration
- 0.5 mg/mL
Submitted references Cholesterol alters mitophagy by impairing optineurin recruitment and lysosomal clearance in Alzheimer's disease.
Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair.
Reprogramming of Amino Acid Transporters to Support Aspartate and Glutamate Dependency Sustains Endocrine Resistance in Breast Cancer.
The BEACH-containing protein WDR81 coordinates p62 and LC3C to promote aggrephagy.
Molecular Determinants of Scaffold-induced Linear Ubiquitinylation of B Cell Lymphoma/Leukemia 10 (Bcl10) during T Cell Receptor and Oncogenic Caspase Recruitment Domain-containing Protein 11 (CARD11) Signaling.
Fucose-specific DC-SIGN signalling directs T helper cell type-2 responses via IKKε- and CYLD-dependent Bcl3 activation.
A quantitative signaling screen identifies CARD11 mutations in the CARD and LATCH domains that induce Bcl10 ubiquitination and human lymphoma cell survival.
Degradation of the antiviral component ARGONAUTE1 by the autophagy pathway.
Polyubiquitin linkage profiles in three models of proteolytic stress suggest the etiology of Alzheimer disease.
Regulation of Abro1/KIAA0157 during myocardial infarction and cell death reveals a novel cardioprotective mechanism for Lys63-specific deubiquitination.
Roca-Agujetas V, Barbero-Camps E, de Dios C, Podlesniy P, Abadin X, Morales A, Marí M, Trullàs R, Colell A
Molecular neurodegeneration 2021 Mar 8;16(1):15
Molecular neurodegeneration 2021 Mar 8;16(1):15
Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair.
Nakazawa Y, Hara Y, Oka Y, Komine O, van den Heuvel D, Guo C, Daigaku Y, Isono M, He Y, Shimada M, Kato K, Jia N, Hashimoto S, Kotani Y, Miyoshi Y, Tanaka M, Sobue A, Mitsutake N, Suganami T, Masuda A, Ohno K, Nakada S, Mashimo T, Yamanaka K, Luijsterburg MS, Ogi T
Cell 2020 Mar 19;180(6):1228-1244.e24
Cell 2020 Mar 19;180(6):1228-1244.e24
Reprogramming of Amino Acid Transporters to Support Aspartate and Glutamate Dependency Sustains Endocrine Resistance in Breast Cancer.
Bacci M, Lorito N, Ippolito L, Ramazzotti M, Luti S, Romagnoli S, Parri M, Bianchini F, Cappellesso F, Virga F, Gao Q, Simões BM, Marangoni E, Martin LA, Comito G, Ferracin M, Giannoni E, Mazzone M, Chiarugi P, Morandi A
Cell reports 2019 Jul 2;28(1):104-118.e8
Cell reports 2019 Jul 2;28(1):104-118.e8
The BEACH-containing protein WDR81 coordinates p62 and LC3C to promote aggrephagy.
Liu X, Li Y, Wang X, Xing R, Liu K, Gan Q, Tang C, Gao Z, Jian Y, Luo S, Guo W, Yang C
The Journal of cell biology 2017 May 1;216(5):1301-1320
The Journal of cell biology 2017 May 1;216(5):1301-1320
Molecular Determinants of Scaffold-induced Linear Ubiquitinylation of B Cell Lymphoma/Leukemia 10 (Bcl10) during T Cell Receptor and Oncogenic Caspase Recruitment Domain-containing Protein 11 (CARD11) Signaling.
Yang YK, Yang C, Chan W, Wang Z, Deibel KE, Pomerantz JL
The Journal of biological chemistry 2016 Dec 9;291(50):25921-25936
The Journal of biological chemistry 2016 Dec 9;291(50):25921-25936
Fucose-specific DC-SIGN signalling directs T helper cell type-2 responses via IKKε- and CYLD-dependent Bcl3 activation.
Gringhuis SI, Kaptein TM, Wevers BA, Mesman AW, Geijtenbeek TB
Nature communications 2014 May 28;5:3898
Nature communications 2014 May 28;5:3898
A quantitative signaling screen identifies CARD11 mutations in the CARD and LATCH domains that induce Bcl10 ubiquitination and human lymphoma cell survival.
Chan W, Schaffer TB, Pomerantz JL
Molecular and cellular biology 2013 Jan;33(2):429-43
Molecular and cellular biology 2013 Jan;33(2):429-43
Degradation of the antiviral component ARGONAUTE1 by the autophagy pathway.
Derrien B, Baumberger N, Schepetilnikov M, Viotti C, De Cillia J, Ziegler-Graff V, Isono E, Schumacher K, Genschik P
Proceedings of the National Academy of Sciences of the United States of America 2012 Sep 25;109(39):15942-6
Proceedings of the National Academy of Sciences of the United States of America 2012 Sep 25;109(39):15942-6
Polyubiquitin linkage profiles in three models of proteolytic stress suggest the etiology of Alzheimer disease.
Dammer EB, Na CH, Xu P, Seyfried NT, Duong DM, Cheng D, Gearing M, Rees H, Lah JJ, Levey AI, Rush J, Peng J
The Journal of biological chemistry 2011 Mar 25;286(12):10457-65
The Journal of biological chemistry 2011 Mar 25;286(12):10457-65
Regulation of Abro1/KIAA0157 during myocardial infarction and cell death reveals a novel cardioprotective mechanism for Lys63-specific deubiquitination.
Cilenti L, Balakrishnan MP, Wang XL, Ambivero C, Sterlicchi M, del Monte F, Ma XL, Zervos AS
Journal of molecular and cellular cardiology 2011 Apr;50(4):652-61
Journal of molecular and cellular cardiology 2011 Apr;50(4):652-61
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- In vitro linked and purified polyubiquitin chains were analyzed by SDS-PAGE followed by immunoblotting with Anti-Human Ub-K63 Purified. The antibody detects lysine-63-linked chains (lane 1), but does not detect lysine-48-linked chains (lane 2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL