MA5-24199

antibody from Invitrogen Antibodies

Targeting: CA5A CA5, CAV, CAVA

Western blot (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24199

Provider

Invitrogen Antibodies

Product name

Carbonic Anhydrase VA/VB Monoclonal Antibody (487908)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In Western blots, no cross-reactivity with recombinant human CA1, 4, or CA6-14 is observed. Reconstitute at 0.5 mg/mL in sterile PBS.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

487908

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Carbonic Anhydrase VA/VB in immersion fixed paraffin-embedded sections of human liver. Samples were incubated in Carbonic Anhydrase VA/VB monoclonal antibody (Product # MA5-24199) using a dilution of 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of hepatocytes.