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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-38418 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-PKC Pan (Thr497) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects PRKCA, PRKCB, PRKCD, PRKCE, PRKCG, PRKCH, PRKCQ and PRKCZ
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-PKC pan pThr497 in extracts from NIH-3T3 cells using a Phospho-PKC pan pThr497 polyclonal antibody (Product # PA5-38418).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Phospho-PKC Pan (Thr497) Polyclonal Antibody (Product # PA5-38418) and a 80 kDa band corresponding to Phospho-PKC Pan (Thr497) was observed in HEK 293 cells upon treatment with PMA. Whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), HEK-293 treated with PMA (200 nM) for 30 minutes (Lane 2) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-PKC pan pThr497 in HepG2 cells using a Phospho-PKC pan pThr497 polyclonal antibody (Product # PA5-38418).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-PKC pan pThr497 in HepG2 cells using a Phospho-PKC pan pThr497 polyclonal antibody (Product # PA5-38418).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-PKC pan pThr497 in paraffin-embedded human breast carcinoma using a Phospho-PKC pan pThr497 polyclonal antibody (Product # PA5-38418).
