Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- AM06659SU-N - Provider product page
- Provider
- OriGene
- Product name
- IRAK4 mouse monoclonal antibody, clone 2H9, Ascites
- Antibody type
- Monoclonal
- Description
- IRAK4 mouse monoclonal antibody, clone 2H9, Ascites
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- IRAK4
- Isotype
- IgG
- Antibody clone number
- 2H9
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using IRAK4 mAb against human IRAK4 (AA: 21-198) recombinant protein. (Expected MW is 45.4 kDa)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using IRAK4 mouse mAb against THP-1 (1), Hela (2), K562 (3), MCF-7 (4), RAW264.7 (5), Jurkat (6) and Cos7 (7) cell lysate.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using IRAK4 mouse mAb with DAB staining.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human kidney cancer tissues using IRAK4 mouse mAb with DAB staining.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells using IRAK4 mouse mAb (blue) and negative control (red).
- Validation comment
- FC