MA5-12429

antibody from Invitrogen Antibodies

Targeting: KRT6B KRTL1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-12429

Provider

Invitrogen Antibodies

Product name

Cytokeratin 5/6 Monoclonal Antibody (D5/I6 B4)

Antibody type

Monoclonal

Antigen

Other

Description

MA5-12429 targets Cytokeratin 5/6 in IHC (P) applications and shows reactivity with Human samples. The MA5-12429 immunogen is isolated cytokeratin 5.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

D5/I6 B4

Vial size

500 µL

Concentration

Conc. Not Determined

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed using whole cell extracts (30 µg) of A-375 (Lane 1) and NIH/3T3 (Lane 2). The blots were probed with Anti-Cytokeratin5 + Cytokeratin 6 Mouse Monoclonal Antibody (Product # MA5-12429, 1:250 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A 62 kDa band corresponding to Cytokeratin 5 + Cytokeratin 6 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human mesothelioma stained with Keratin 5/6 using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 LCOs recapitulate the characteristics of the original tissues. a - d H&E-stained and IHC-stained images of LCOs and their original LC tissues. The enlarged images in blue boxes of b showed cytoplasmic keratinisation of individual squamous carcinoma cells. Scale bars, 100 mum. e H&E and IHC-stained images of an adenosquamous carcinoma organoid (upper panel). Individual organoids expressed either adenocarcinoma marker (CK7) or squamous cell carcinoma markers (CK5/6 or p63). Some single organoids showed mixed cell pattern composed of CK7+ and CK7- cells, CK5/6+ and CK5/6- cells, and p63+ and p63- cells. Scale bar, 100 mum. Bright field microscopy and immunofluorescence (IF) images of an adenosqumaous carcinoma organoid originated from a single cell (lower panel). The immunofluorescence staining was performed at Day 18 after seeding. The organoid from a single cell was composed of p63+/CK7- cells and p63-/CK7+ cells. The red arrow indicated a single cell seeded in a micro-well. Scale bar in bright-field microscopy images, 100 mum. Scale bar in IF images, 20 mum