Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [6]
- Flow cytometry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX84850 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84850, RRID:AB_10730051
- Product name
- ATP5B antibody [1F7]
- Antibody type
- Monoclonal
- Reactivity
- Human, Canine, Simian
- Host
- Mouse
- Storage
- For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-ATP5B mouse monoclonal antibody (GTX84850) immunofluorescent staining of COS7 cells transiently transfected with ATP5B
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-ATP5B mouse monoclonal antibody (GTX84850).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (GTX84850, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84850, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84850, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84850, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84850, Dilution 1:50)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84850, Dilution 1:50)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY ATP5B
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells, using anti-ATP5B antibody(GTX84850),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-ATP5B antibody(GTX84850),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).