GTX84850

antibody from GeneTex

Targeting: ATP5F1B ATP5B, ATPSB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX84850

Provider

GeneTex

Proper citation

GeneTex Cat#GTX84850, RRID:AB_10730051

Product name

ATP5B antibody [1F7]

Antibody type

Monoclonal

Reactivity

Human, Canine, Simian

Host

Mouse

Storage

For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

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GeneTex (provider)

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Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP5B monoclonal antibody.

Supportive validation

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GeneTex (provider)

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Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5B.

Immunocytochemistry

Supportive validation

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GeneTex (provider)

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Experimental details

Anti-ATP5B mouse monoclonal antibody (GTX84850) immunofluorescent staining of COS7 cells transiently transfected with ATP5B

Supportive validation

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GeneTex (provider)

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Experimental details

Immunofluorescent staining of HepG2 cells using anti-ATP5B mouse monoclonal antibody (GTX84850).

Immunohistochemistry

Supportive validation

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GeneTex (provider)

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Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-ATP5B mouse monoclonal antibody. (GTX84850, Dilution 1:50)

Supportive validation

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GeneTex (provider)

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Experimental details

Immunohistochemical staining of paraffin-embedded liver tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84850, Dilution 1:50)

Supportive validation

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GeneTex (provider)

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Experimental details

Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84850, Dilution 1:50)

Supportive validation

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GeneTex (provider)

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Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84850, Dilution 1:50)

Supportive validation

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GeneTex (provider)

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Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84850, Dilution 1:50)

Supportive validation

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GeneTex (provider)

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Experimental details

Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-ATP5Bmouse monoclonal antibody. (GTX84850, Dilution 1:50)

Flow cytometry

Supportive validation

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GeneTex (provider)

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Experimental details

HEK293T cells transfected with either pCMV6-ENTRY ATP5B

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometric analysis of Hela cells, using anti-ATP5B antibody(GTX84850),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometric analysis of Jurkat cells, using anti-ATP5B antibody(GTX84850),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).