Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX25432 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX25432, RRID:AB_373939
- Product name
- ATP5B antibody [4.3E8.D10]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of ATP Synthase beta from rat neuronal/glial cell extract.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Indirect immunofluorescence staining of rat neuronal/glial cell culture.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of ATP Synthase beta in THP-1 cells. Antigen-antibody complexes were formed by incubating 500ug of THP-1 whole cell lysate (in 500ul volume) with 5ul of ATP Synthase beta antibody [4.3E8.D10] overnight at 4¢XC. The immune complexes were captured on 30ul of protein G agarose, washed extensively, and eluted. Samples were resolved on an 8% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with ATP Synthase beta antibody [4.3E8.D10] at a dilution of 1:1000 overnight at 4¢XC, washed in TBST, and probed with an HRP-conjugated secondary antibody. Chemiluminescent detection was performed.