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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP17895PU-N - Provider product page
- Provider
- OriGene
- Product name
- ATP5F1B (Center) rabbit polyclonal antibody
- Antibody type
- Polyclonal
- Description
- ATP5F1B (Center) rabbit polyclonal antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ATP5F1B
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.398 mg/ml.
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- A: ?Western blot analysis of ATP5B Antibody (Center) pre-incubated without (lane 1) and with (lane 2) blocking peptide in WiDr cell line lysate. ATP5B (arrow) was detected using the purified Pab (1:60/250 dilution).? B: Western blot analysis of ATP5B (arrow) using Rabbit polyclonal ATP5B Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATP5B gene (Lane 2).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with ATP5B Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of WiDr cells (right histogram) compared to a negative control cell using ATP5B Antibody (Center) (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
