MA1-930-A647

antibody from Invitrogen Antibodies

Targeting: ATP5F1B ATP5B, ATPSB

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-930-A647

Provider

Invitrogen Antibodies

Product name

ATP Synthase beta Monoclonal Antibody (4.3E8.D10), Alexa Fluor™ 647

Antibody type

Monoclonal

Antigen

Other

Description

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Red dye

Isotype

IgG

Antibody clone number

4.3E8.D10

Vial size

50 µL

Concentration

1 mg/mL

Storage

4° C, store in dark, DO NOT FREEZE!

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ATP Synthase Beta (red) in HeLa cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a ATP Synthase beta mouse monoclonal antibody, AlexaFluor 647 conjugate (Product # MA1-930-A647), at a concentration of 5 µg/mL in blocking buffer for at least 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Conjugate

Red dye