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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunoprecipitation [2]
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- Product number
- NB600-1171 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-1171, RRID:AB_10002846
- Product name
- Mouse Monoclonal ATPB Antibody
- Antibody type
- Monoclonal
- Description
- Immunogen affinity purified. Detects the beta subunit of ATP synthase from mouse rat and human samples. This is useful as a mitochondrial marker.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100ug
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references CHCHD2 accumulates in distressed mitochondria and facilitates oligomerization of CHCHD10.
Chronic exposure of adult rats to low doses of methylmercury induced a state of metabolic deficit in the somatosensory cortex.
The β-subunit of ATP synthase is involved in cellular uptake and resecretion of apoA-I but does not control apoA-I-induced lipid efflux in adipocytes.
Huang X, Wu BP, Nguyen D, Liu YT, Marani M, Hench J, Bénit P, Kozjak-Pavlovic V, Rustin P, Frank S, Narendra DP
Human molecular genetics 2018 Nov 15;27(22):3881-3900
Human molecular genetics 2018 Nov 15;27(22):3881-3900
Chronic exposure of adult rats to low doses of methylmercury induced a state of metabolic deficit in the somatosensory cortex.
Kong HK, Wong MH, Chan HM, Lo SC
Journal of proteome research 2013 Nov 1;12(11):5233-45
Journal of proteome research 2013 Nov 1;12(11):5233-45
The β-subunit of ATP synthase is involved in cellular uptake and resecretion of apoA-I but does not control apoA-I-induced lipid efflux in adipocytes.
Howard AD, Verghese PB, Arrese EL, Soulages JL
Molecular and cellular biochemistry 2011 Feb;348(1-2):155-64
Molecular and cellular biochemistry 2011 Feb;348(1-2):155-64
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of ATP Synthase beta was performed on THP-1 cells. Antigen-antibody complexes were formed by incubating 500ug of THP-1 whole cell lysate (in 500ul volume) with 5ul of an ATP Synthase beta monoclonal antibody overnight at 4C. The immune complexes were captured on 30ul of protein G agarose, washed extensively, and eluted with 6X Laemmli non-reducing sample buffer. Samples were resolved on an 8% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with an ATP Synthase beta monoclonal antibody at a dilution of 1:1000 overnight at 4C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG secondary at a dilution of 1:40,000 for 1 hour at room temperature. Chemiluminescent detection was performed using ECL substrate. Data courtesy of the Innovators Program.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ATPB Antibody (4.3E8.D10) [NB600-1171] - Analysis of rat neuronal/glial cell extract.
