PA5-36865

antibody from Invitrogen Antibodies

Targeting: PAK5 KIAA1264, PAK7

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA5-36865

Provider

Invitrogen Antibodies

Product name

Phospho-PAK4/6/7 (Ser474) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 72 kDa (PAK4), 82 kDa (PAK6) and 90 (PAK7). Purity is >95% by SDS-PAGE.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-PAK4+PAK5+PAK6 pSer474 using Phospho-PAK4+PAK5+PAK6 pSer474 polyclonal antibody (Product # PA5-36865) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with EGF (0.1 ng/mL, 30 min), Lane 2: Raw264.7 cell lysate treated with EGF (E0.1 ng/mL, 30 min), Lane 3: PC12 cell lysate treated with EGF (0.1 ng/mL, 30 min).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-PAK4/6/7 (Ser474) in Lane 1: PC3 whole cell lysate (40 µg), Lane 2: HCT116 whole cell lysate (40 µg), Lane 3: MCF-7 whole cell lysate (40 µg). Samples were incubated with Phospho-PAK4/6/7 (Ser474) polyclonal antibody (Product # PA5-36865) at a dilution of 1:500.