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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-17185 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Brachyury Monoclonal Antibody (1H9A2)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17185 targets Brachyury protein T in indirect ELISA, FACS, IHC and WB applications and shows reactivity with Human samples. The MA5-17185 immunogen is purified recombinant fragment of human T (amino acids: 257-309 ) expressed in E. Coli. MA5-17185 detects Brachyury protein T which has a predicted molecular weight of approximately 47.4kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1H9A2
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Brachyury protein T using a Brachyury protein T monoclonal antibody (Product # MA5-17185) against a human T recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Brachyury protein T using a Brachyury protein T monoclonal antibody (Product # MA5-17185) against a human T recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Brachyury protein T using Brachyury protein T monoclonal antibody (Product # MA5-17185) in HEK293 (1) and T (AA: 257-309) human IgG Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Brachyury protein T using Brachyury protein T monoclonal antibody (Product # MA5-17185) in Raji (1), and Jurkat (2) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Brachyury protein T monoclonal antibody (Product # MA5-17185) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using Brachyury protein T monoclonal antibody (Product # MA5-17185) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using Brachyury protein T monoclonal antibody (Product # MA5-17185) (green) and negative control (red).
