Antibody data
- Antibody Data
- Antigen structure
- References [56]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
Reference
Comment
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- Product number
- AF2085 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Brachyury Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human TBX-6 is observed.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O15178
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Complementary Activity of ETV5, RBPJ, and TCF3 Drives Formative Transition from Naive Pluripotency.
Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells.
Generation of an induced pluripotent stem cell line (FRIMOi002-A) from a retinitis pigmentosa patient carrying compound heterozygous mutations in USH2A gene.
Establishment and characterization of an iPSC line (FRIMOi001-A) derived from a retinitis pigmentosa patient carrying PDE6A mutations.
Establishment of an induced pluripotent stem cell line (FRIMOi005-A) derived from a retinitis pigmentosa patient carrying a dominant mutation in RHO gene.
Generation of Spinocerebellar Ataxia Type 2 induced pluripotent stem cell lines, CHOPi002-A and CHOPi003-A, from patients with abnormal CAG repeats in the coding region of the ATXN2 gene.
Generation of two iPS cell lines (FRIMOi003-A and FRIMOi004-A) derived from Stargardt patients carrying ABCA4 compound heterozygous mutations.
User-defined morphogen patterning for directing human cell fate stratification.
Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation.
In vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cells.
Lymphoblast-derived integration-free ISRM-CON9 iPS cell line from a 75year old female.
Short-term retinoic acid treatment sustains pluripotency and suppresses differentiation of human induced pluripotent stem cells.
Modeling signaling-dependent pluripotency with Boolean logic to predict cell fate transitions.
Generation of a human Ocular Albinism type 1 iPSC line, SEIi001-A, with a mutation in GPR143.
Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel.
Generation of a human Juvenile myelomonocytic leukemia iPSC line, CHOPi001-A, with a mutation in CBL.
Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation.
ADAM17 is required for EGF-R-induced intestinal tumors via IL-6 trans-signaling.
The lipodystrophic hotspot lamin A p.R482W mutation deregulates the mesodermal inducer T/Brachyury and early vascular differentiation gene networks.
The circular RNA circBIRC6 participates in the molecular circuitry controlling human pluripotency.
Guided self-organization and cortical plate formation in human brain organoids.
CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells.
The p53 Family Coordinates Wnt and Nodal Inputs in Mesendodermal Differentiation of Embryonic Stem Cells.
Jak1/Stat3 signaling acts as a positive regulator of pluripotency in chicken pre-gastrula embryos.
Real-time and non-invasive monitoring of embryonic stem cell survival during the development of embryoid bodies with smart nanosensor.
A Gradient of Glycolytic Activity Coordinates FGF and Wnt Signaling during Elongation of the Body Axis in Amniote Embryos.
In Vitro Micropatterned Human Pluripotent Stem Cell Test (µP-hPST) for Morphometric-Based Teratogen Screening.
Visualization of the Epiblast and Visceral Endodermal Cells Using Fgf5-P2A-Venus BAC Transgenic Mice and Epiblast Stem Cells.
Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells.
ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation.
Dynamic Heterogeneity of Brachyury in Mouse Epiblast Stem Cells Mediates Distinct Response to Extrinsic Bone Morphogenetic Protein (BMP) Signaling.
PI3K/mTORC2 regulates TGF-β/Activin signalling by modulating Smad2/3 activity via linker phosphorylation.
An alternative pluripotent state confers interspecies chimaeric competency.
Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation.
Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes.
T-box transcription factor brachyury is associated with prostate cancer progression and aggressiveness.
Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors.
Insulin-like growth factor promotes cardiac lineage induction in vitro by selective expansion of early mesoderm.
SRF is essential for mesodermal cell migration during elongation of the embryonic body axis.
In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity.
Induction of pluripotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm.
The role of Hath6, a newly identified shear-stress-responsive transcription factor, in endothelial cell differentiation and function.
Oct4 is required ~E7.5 for proliferation in the primitive streak.
Genomic targets of Brachyury (T) in differentiating mouse embryonic stem cells.
The endothelial cell line bEnd.3 maintains human pluripotent stem cells.
A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3.
Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells.
A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells.
TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal.
Creation of engineered human embryonic stem cell lines using phiC31 integrase.
Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells.
A porous photocurable elastomer for cell encapsulation and culture.
Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed.
Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking.
Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells.
Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions.
Kalkan T, Bornelöv S, Mulas C, Diamanti E, Lohoff T, Ralser M, Middelkamp S, Lombard P, Nichols J, Smith A
Cell stem cell 2019 May 2;24(5):785-801.e7
Cell stem cell 2019 May 2;24(5):785-801.e7
Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells.
Wei X, Guo J, Li Q, Jia Q, Jing Q, Li Y, Zhou B, Chen J, Gao S, Zhang X, Jia M, Niu C, Yang W, Zhi X, Wang X, Yu D, Bai L, Wang L, Na J, Zou Y, Zhang J, Zhang S, Meng D
Science advances 2019 Mar;5(3):eaau7887
Science advances 2019 Mar;5(3):eaau7887
Generation of an induced pluripotent stem cell line (FRIMOi002-A) from a retinitis pigmentosa patient carrying compound heterozygous mutations in USH2A gene.
Riera M, Patel A, Corcostegui B, Chang S, Corneo B, Sparrow JR, Pomares E
Stem cell research 2019 Mar;35:101386
Stem cell research 2019 Mar;35:101386
Establishment and characterization of an iPSC line (FRIMOi001-A) derived from a retinitis pigmentosa patient carrying PDE6A mutations.
Riera M, Patel A, Corcostegui B, Chang S, Sparrow JR, Pomares E, Corneo B
Stem cell research 2019 Mar;35:101385
Stem cell research 2019 Mar;35:101385
Establishment of an induced pluripotent stem cell line (FRIMOi005-A) derived from a retinitis pigmentosa patient carrying a dominant mutation in RHO gene.
Domingo-Prim J, Riera M, Burés-Jelstrup A, Corcostegui B, Pomares E
Stem cell research 2019 Jul;38:101468
Stem cell research 2019 Jul;38:101468
Generation of Spinocerebellar Ataxia Type 2 induced pluripotent stem cell lines, CHOPi002-A and CHOPi003-A, from patients with abnormal CAG repeats in the coding region of the ATXN2 gene.
Maguire JA, Gagne AL, Gonzalez-Alegre P, Davidson BL, Shakkottai V, Gadue P, French DL
Stem cell research 2019 Jan;34:101361
Stem cell research 2019 Jan;34:101361
Generation of two iPS cell lines (FRIMOi003-A and FRIMOi004-A) derived from Stargardt patients carrying ABCA4 compound heterozygous mutations.
Riera M, Patel A, Burés-Jelstrup A, Corcostegui B, Chang S, Pomares E, Corneo B, Sparrow JR
Stem cell research 2019 Apr;36:101389
Stem cell research 2019 Apr;36:101389
User-defined morphogen patterning for directing human cell fate stratification.
Regier MC, Tokar JJ, Warrick JW, Pabon L, Berthier E, Beebe DJ, Stevens KR
Scientific reports 2019 Apr 23;9(1):6433
Scientific reports 2019 Apr 23;9(1):6433
Lineage-Specific Chiral Biases of Human Embryonic Stem Cells during Differentiation.
Worley KE, Chin AS, Wan LQ
Stem cells international 2018;2018:1848605
Stem cells international 2018;2018:1848605
In vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cells.
Harrison SE, Sozen B, Zernicka-Goetz M
Nature protocols 2018 Jul;13(7):1586-1602
Nature protocols 2018 Jul;13(7):1586-1602
Lymphoblast-derived integration-free ISRM-CON9 iPS cell line from a 75year old female.
Martins S, Bohndorf M, Schröter F, Assar F, Wruck W, Sleegers K, Van Broeckhoven C, Adjaye J
Stem cell research 2018 Jan;26:76-79
Stem cell research 2018 Jan;26:76-79
Short-term retinoic acid treatment sustains pluripotency and suppresses differentiation of human induced pluripotent stem cells.
De Angelis MT, Parrotta EI, Santamaria G, Cuda G
Cell death & disease 2018 Jan 5;9(1):6
Cell death & disease 2018 Jan 5;9(1):6
Modeling signaling-dependent pluripotency with Boolean logic to predict cell fate transitions.
Yachie-Kinoshita A, Onishi K, Ostblom J, Langley MA, Posfai E, Rossant J, Zandstra PW
Molecular systems biology 2018 Jan 29;14(1):e7952
Molecular systems biology 2018 Jan 29;14(1):e7952
Generation of a human Ocular Albinism type 1 iPSC line, SEIi001-A, with a mutation in GPR143.
Baulier E, Garcia Diaz A, Corneo B, Farber DB
Stem cell research 2018 Dec;33:274-277
Stem cell research 2018 Dec;33:274-277
Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel.
Gao X, Yourick JJ, Sprando RL
Stem cell research 2018 Aug;31:193-196
Stem cell research 2018 Aug;31:193-196
Generation of a human Juvenile myelomonocytic leukemia iPSC line, CHOPi001-A, with a mutation in CBL.
Gagne AL, Maguire JA, Gandre-Babbe S, Chou ST, Tasian SK, Loh ML, Weiss MJ, Gadue P, French DL
Stem cell research 2018 Aug;31:157-160
Stem cell research 2018 Aug;31:157-160
Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation.
Corsini NS, Peer AM, Moeseneder P, Roiuk M, Burkard TR, Theussl HC, Moll I, Knoblich JA
Cell stem cell 2018 Apr 5;22(4):543-558.e12
Cell stem cell 2018 Apr 5;22(4):543-558.e12
ADAM17 is required for EGF-R-induced intestinal tumors via IL-6 trans-signaling.
Schmidt S, Schumacher N, Schwarz J, Tangermann S, Kenner L, Schlederer M, Sibilia M, Linder M, Altendorf-Hofmann A, Knösel T, Gruber ES, Oberhuber G, Bolik J, Rehman A, Sinha A, Lokau J, Arnold P, Cabron AS, Zunke F, Becker-Pauly C, Preaudet A, Nguyen P, Huynh J, Afshar-Sterle S, Chand AL, Westermann J, Dempsey PJ, Garbers C, Schmidt-Arras D, Rosenstiel P, Putoczki T, Ernst M, Rose-John S
The Journal of experimental medicine 2018 Apr 2;215(4):1205-1225
The Journal of experimental medicine 2018 Apr 2;215(4):1205-1225
The lipodystrophic hotspot lamin A p.R482W mutation deregulates the mesodermal inducer T/Brachyury and early vascular differentiation gene networks.
Briand N, Guénantin AC, Jeziorowska D, Shah A, Mantecon M, Capel E, Garcia M, Oldenburg A, Paulsen J, Hulot JS, Vigouroux C, Collas P
Human molecular genetics 2018 Apr 15;27(8):1447-1459
Human molecular genetics 2018 Apr 15;27(8):1447-1459
The circular RNA circBIRC6 participates in the molecular circuitry controlling human pluripotency.
Yu CY, Li TC, Wu YY, Yeh CH, Chiang W, Chuang CY, Kuo HC
Nature communications 2017 Oct 27;8(1):1149
Nature communications 2017 Oct 27;8(1):1149
Guided self-organization and cortical plate formation in human brain organoids.
Lancaster MA, Corsini NS, Wolfinger S, Gustafson EH, Phillips AW, Burkard TR, Otani T, Livesey FJ, Knoblich JA
Nature biotechnology 2017 Jul;35(7):659-666
Nature biotechnology 2017 Jul;35(7):659-666
CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells.
Wong KG, Ryan SD, Ramnarine K, Rosen SA, Mann SE, Kulick A, De Stanchina E, Müller FJ, Kacmarczyk TJ, Zhang C, Betel D, Tomishima MJ
Stem cell reports 2017 Jul 11;9(1):355-365
Stem cell reports 2017 Jul 11;9(1):355-365
The p53 Family Coordinates Wnt and Nodal Inputs in Mesendodermal Differentiation of Embryonic Stem Cells.
Wang Q, Zou Y, Nowotschin S, Kim SY, Li QV, Soh CL, Su J, Zhang C, Shu W, Xi Q, Huangfu D, Hadjantonakis AK, Massagué J
Cell stem cell 2017 Jan 5;20(1):70-86
Cell stem cell 2017 Jan 5;20(1):70-86
Jak1/Stat3 signaling acts as a positive regulator of pluripotency in chicken pre-gastrula embryos.
Nakanoh S, Fuse N, Tadokoro R, Takahashi Y, Agata K
Developmental biology 2017 Jan 1;421(1):43-51
Developmental biology 2017 Jan 1;421(1):43-51
Real-time and non-invasive monitoring of embryonic stem cell survival during the development of embryoid bodies with smart nanosensor.
Fu J, Wiraja C, Chong R, Xu C, Wang DA
Acta biomaterialia 2017 Feb;49:358-367
Acta biomaterialia 2017 Feb;49:358-367
A Gradient of Glycolytic Activity Coordinates FGF and Wnt Signaling during Elongation of the Body Axis in Amniote Embryos.
Oginuma M, Moncuquet P, Xiong F, Karoly E, Chal J, Guevorkian K, Pourquié O
Developmental cell 2017 Feb 27;40(4):342-353.e10
Developmental cell 2017 Feb 27;40(4):342-353.e10
In Vitro Micropatterned Human Pluripotent Stem Cell Test (µP-hPST) for Morphometric-Based Teratogen Screening.
Xing J, Cao Y, Yu Y, Li H, Song Z, Yu H
Scientific reports 2017 Aug 17;7(1):8491
Scientific reports 2017 Aug 17;7(1):8491
Visualization of the Epiblast and Visceral Endodermal Cells Using Fgf5-P2A-Venus BAC Transgenic Mice and Epiblast Stem Cells.
Khoa le TP, Azami T, Tsukiyama T, Matsushita J, Tsukiyama-Fujii S, Takahashi S, Ema M
PloS one 2016;11(7):e0159246
PloS one 2016;11(7):e0159246
Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells.
Chang WF, Hwu YM, Xu J, Lin CJ, Wang SW, Cheng AS, Lu J, Lu CH, Sung LY
PloS one 2016;11(11):e0165715
PloS one 2016;11(11):e0165715
ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation.
Maldonado M, Luu RJ, Ramos ME, Nam J
Stem cell research 2016 Sep;17(2):222-227
Stem cell research 2016 Sep;17(2):222-227
Dynamic Heterogeneity of Brachyury in Mouse Epiblast Stem Cells Mediates Distinct Response to Extrinsic Bone Morphogenetic Protein (BMP) Signaling.
Song L, Chen J, Peng G, Tang K, Jing N
The Journal of biological chemistry 2016 Jul 15;291(29):15212-25
The Journal of biological chemistry 2016 Jul 15;291(29):15212-25
PI3K/mTORC2 regulates TGF-β/Activin signalling by modulating Smad2/3 activity via linker phosphorylation.
Yu JS, Ramasamy TS, Murphy N, Holt MK, Czapiewski R, Wei SK, Cui W
Nature communications 2015 May 22;6:7212
Nature communications 2015 May 22;6:7212
An alternative pluripotent state confers interspecies chimaeric competency.
Wu J, Okamura D, Li M, Suzuki K, Luo C, Ma L, He Y, Li Z, Benner C, Tamura I, Krause MN, Nery JR, Du T, Zhang Z, Hishida T, Takahashi Y, Aizawa E, Kim NY, Lajara J, Guillen P, Campistol JM, Esteban CR, Ross PJ, Saghatelian A, Ren B, Ecker JR, Izpisua Belmonte JC
Nature 2015 May 21;521(7552):316-21
Nature 2015 May 21;521(7552):316-21
Lineage tracing of neuromesodermal progenitors reveals novel Wnt-dependent roles in trunk progenitor cell maintenance and differentiation.
Garriock RJ, Chalamalasetty RB, Kennedy MW, Canizales LC, Lewandoski M, Yamaguchi TP
Development (Cambridge, England) 2015 May 1;142(9):1628-38
Development (Cambridge, England) 2015 May 1;142(9):1628-38
Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes.
Maury Y, Côme J, Piskorowski RA, Salah-Mohellibi N, Chevaleyre V, Peschanski M, Martinat C, Nedelec S
Nature biotechnology 2015 Jan;33(1):89-96
Nature biotechnology 2015 Jan;33(1):89-96
T-box transcription factor brachyury is associated with prostate cancer progression and aggressiveness.
Pinto F, Pértega-Gomes N, Pereira MS, Vizcaíno JR, Monteiro P, Henrique RM, Baltazar F, Andrade RP, Reis RM
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Sep 15;20(18):4949-61
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Sep 15;20(18):4949-61
Distinct Wnt-driven primitive streak-like populations reflect in vivo lineage precursors.
Tsakiridis A, Huang Y, Blin G, Skylaki S, Wymeersch F, Osorno R, Economou C, Karagianni E, Zhao S, Lowell S, Wilson V
Development (Cambridge, England) 2014 Mar;141(6):1209-21
Development (Cambridge, England) 2014 Mar;141(6):1209-21
Insulin-like growth factor promotes cardiac lineage induction in vitro by selective expansion of early mesoderm.
Engels MC, Rajarajan K, Feistritzer R, Sharma A, Nielsen UB, Schalij MJ, de Vries AA, Pijnappels DA, Wu SM
Stem cells (Dayton, Ohio) 2014 Jun;32(6):1493-502
Stem cells (Dayton, Ohio) 2014 Jun;32(6):1493-502
SRF is essential for mesodermal cell migration during elongation of the embryonic body axis.
Schwartz B, Marks M, Wittler L, Werber M, Währisch S, Nordheim A, Herrmann BG, Grote P
Mechanisms of development 2014 Aug;133:23-35
Mechanisms of development 2014 Aug;133:23-35
In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity.
Gouti M, Tsakiridis A, Wymeersch FJ, Huang Y, Kleinjung J, Wilson V, Briscoe J
PLoS biology 2014 Aug;12(8):e1001937
PLoS biology 2014 Aug;12(8):e1001937
Induction of pluripotency in human somatic cells via a transient state resembling primitive streak-like mesendoderm.
Takahashi K, Tanabe K, Ohnuki M, Narita M, Sasaki A, Yamamoto M, Nakamura M, Sutou K, Osafune K, Yamanaka S
Nature communications 2014 Apr 24;5:3678
Nature communications 2014 Apr 24;5:3678
The role of Hath6, a newly identified shear-stress-responsive transcription factor, in endothelial cell differentiation and function.
Fang F, Wasserman SM, Torres-Vazquez J, Weinstein B, Cao F, Li Z, Wilson KD, Yue W, Wu JC, Xie X, Pei X
Journal of cell science 2014 Apr 1;127(Pt 7):1428-40
Journal of cell science 2014 Apr 1;127(Pt 7):1428-40
Oct4 is required ~E7.5 for proliferation in the primitive streak.
DeVeale B, Brokhman I, Mohseni P, Babak T, Yoon C, Lin A, Onishi K, Tomilin A, Pevny L, Zandstra PW, Nagy A, van der Kooy D
PLoS genetics 2013 Nov;9(11):e1003957
PLoS genetics 2013 Nov;9(11):e1003957
Genomic targets of Brachyury (T) in differentiating mouse embryonic stem cells.
Evans AL, Faial T, Gilchrist MJ, Down T, Vallier L, Pedersen RA, Wardle FC, Smith JC
PloS one 2012;7(3):e33346
PloS one 2012;7(3):e33346
The endothelial cell line bEnd.3 maintains human pluripotent stem cells.
Joubin K, Richardson A, Novoa N, Tu E, Tomishima MJ
Stem cells and development 2012 Aug 10;21(12):2312-21
Stem cells and development 2012 Aug 10;21(12):2312-21
A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3.
Bone HK, Nelson AS, Goldring CE, Tosh D, Welham MJ
Journal of cell science 2011 Jun 15;124(Pt 12):1992-2000
Journal of cell science 2011 Jun 15;124(Pt 12):1992-2000
Noncanonical Wnt signaling orchestrates early developmental events toward hematopoietic cell fate from human embryonic stem cells.
Vijayaragavan K, Szabo E, Bossé M, Ramos-Mejia V, Moon RT, Bhatia M
Cell stem cell 2009 Mar 6;4(3):248-62
Cell stem cell 2009 Mar 6;4(3):248-62
A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells.
Hansson M, Olesen DR, Peterslund JM, Engberg N, Kahn M, Winzi M, Klein T, Maddox-Hyttel P, Serup P
Developmental biology 2009 Jun 15;330(2):286-304
Developmental biology 2009 Jun 15;330(2):286-304
TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal.
Varelas X, Sakuma R, Samavarchi-Tehrani P, Peerani R, Rao BM, Dembowy J, Yaffe MB, Zandstra PW, Wrana JL
Nature cell biology 2008 Jul;10(7):837-48
Nature cell biology 2008 Jul;10(7):837-48
Creation of engineered human embryonic stem cell lines using phiC31 integrase.
Thyagarajan B, Liu Y, Shin S, Lakshmipathy U, Scheyhing K, Xue H, Ellerström C, Strehl R, Hyllner J, Rao MS, Chesnut JD
Stem cells (Dayton, Ohio) 2008 Jan;26(1):119-26
Stem cells (Dayton, Ohio) 2008 Jan;26(1):119-26
Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells.
Zhang P, Li J, Tan Z, Wang C, Liu T, Chen L, Yong J, Jiang W, Sun X, Du L, Ding M, Deng H
Blood 2008 Feb 15;111(4):1933-41
Blood 2008 Feb 15;111(4):1933-41
A porous photocurable elastomer for cell encapsulation and culture.
Gerecht S, Townsend SA, Pressler H, Zhu H, Nijst CL, Bruggeman JP, Nichol JW, Langer R
Biomaterials 2007 Nov;28(32):4826-35
Biomaterials 2007 Nov;28(32):4826-35
Activin a efficiently specifies definitive endoderm from human embryonic stem cells only when phosphatidylinositol 3-kinase signaling is suppressed.
McLean AB, D'Amour KA, Jones KL, Krishnamoorthy M, Kulik MJ, Reynolds DM, Sheppard AM, Liu H, Xu Y, Baetge EE, Dalton S
Stem cells (Dayton, Ohio) 2007 Jan;25(1):29-38
Stem cells (Dayton, Ohio) 2007 Jan;25(1):29-38
Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking.
De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD
Biotechnology and bioengineering 2007 Aug 15;97(6):1617-25
Biotechnology and bioengineering 2007 Aug 15;97(6):1617-25
Neurogenic effect of vascular endothelial growth factor during germ layer formation of human embryonic stem cells.
Kim BK, Kim SE, Shim JH, Woo DH, Gil JE, Kim SK, Kim JH
FEBS letters 2006 Oct 30;580(25):5869-74
FEBS letters 2006 Oct 30;580(25):5869-74
Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions.
Yao S, Chen S, Clark J, Hao E, Beattie GM, Hayek A, Ding S
Proceedings of the National Academy of Sciences of the United States of America 2006 May 2;103(18):6907-12
Proceedings of the National Academy of Sciences of the United States of America 2006 May 2;103(18):6907-12
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Recombinant Human Brachyury by Western Blot. Western blot shows 10 ng of Recombinant Human Brachyury (amino acids Ser2-Glu202) and Recombinant Human TBX6. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Brachyury at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Brachyury in BG01V Human Stem Cells. Brachyury was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Brachyury in Differentiated Human Embryonic Stem Cells. Brachyury was detected in immersion fixed differentiated human embryonic stem cells using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) for 3 hours at room temperature. Cells were stained (green) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Brachyury in Embryonic Mouse Notochord. Brachyury was detected in immersion fixed frozen sections of embryonic mouse notochord (E9.5) using 10 µg/mL Goat Anti-Human Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) overnight at 4 °C. Tissue was stained with the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Brachyury-regulated Genes by Chromatin Immunoprecipitation. Mesoderm-differentiated BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. Brachyury/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse Brachyury Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2085) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The VEGF promoter was detected by standard PCR.