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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunohistochemistry [2]
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- Product number
- PA5-30690 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TARS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2.
- Concentration
- 0.27 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ThrRS using 50 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a ThrRS polyclonal antibody (Product # PA5-30690) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TARS Polyclonal Antibody detects ThrRS protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TARS Polyclonal Antibody (Product # PA5-30690) diluted by 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), NIH/3T3 (Lane 3), PANC-1 (Lane 4), U-87 MG (Lane5) and MCF7 (Lane 6). The blot was probed with Anti-TARS Polyclonal Antibody (Product # PA5-30690, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 83 kDa band corresponding to TARS was observed across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TARS Polyclonal Antibody (Product # PA5-30690). Sample (30 µg of whole cell lysate). Lane A: A549. 7.5% SDS PAGE. TARS Polyclonal Antibody (Product # PA5-30690) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TARS Polyclonal Antibody detects ThrRS protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TARS Polyclonal Antibody (Product # PA5-30690) diluted by 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of TARS was achieved by transfecting HeLa cells with TARS specific siRNAs (Silencer® select Product # s13793, s13794). Western blot analysis (Fig. a) was performed using whole cell extracts from the TARS knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with TARS Polyclonal Antibody (Product # PA5-30690, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to TARS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TARS Polyclonal Antibody detects ThrRS protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TARS Polyclonal Antibody (Product # PA5-30690) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TARS Polyclonal Antibody detects ThrRS protein at cytoplasm on human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. TARS Polyclonal Antibody (Product # PA5-30690) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
