Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-31088 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Arginase 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, PC-3. Predicted reactivity: Mouse (90%), Rat (87%), Dog (83%), Rabbit (88%), Chicken (81%), Rhesus Monkey (98%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARG2 using 30 µg of A) NT2D1 and B) PC-3 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an ARG2 polyclonal antibody (Product # PA5-31088) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARG2 in 293T whole cell and cytoplasm extracts (30 µg). Samples were separated by 10% SDS-PAGE and the membrane was probed with ARG2 Polyclonal antibody (Product # PA5-31088) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Arginase 2 Polyclonal Antibody (Product # PA5-31088). 293T whole cell and cytoplasm extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Arginase 2 Polyclonal Antibody (Product # PA5-31088) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Arginase 2 was achieved by transfecting LNCaP with Arginase 2 specific siRNA (Silencer® select Product # s1571). Western blot analysis (Fig. a) was performed using whole cell extracts from the Arginase 2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Arginase 2 Polyclonal Antibody (Product # PA5-31088, 1:2000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Arginase 2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Arginase 2 Polyclonal Antibody (Product # PA5-31088) and a 40 kDa band corresponding to Arginase 2 was observed across cell line and tissues tested. HL-60 showed no expression compared to LNCaP. Whole cell extracts (40 µg lysate) of LNCaP (Lane 1), HL-60 (Lane 2), Mouse liver (Lane 3), Mouse kidney (Lane 4), and Rat kidney (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Arginase 2 was performed in HepG2 cells fixed in ice cold MeOH for 5 min. Green: Arginase 2 Polyclonal Antibody (Product # PA5 31088) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Arginase 2 Polyclonal Antibody detects ARG2 protein at mitochondria on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Arginase 2 Polyclonal Antibody (Product # PA5-31088) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U373 xenograft, using ARG2 (Product # PA5-31088) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Arginase 2 Polyclonal Antibody detects ARG2 protein at mitochondria on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Arginase 2 Polyclonal Antibody (Product # PA5-31088) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.