PA5-78282

antibody from Invitrogen Antibodies

Targeting: LRRFIP1 FLAP-1, FLIIAP1, GCF-2, HUFI-1, TRIP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78282

Provider

Invitrogen Antibodies

Product name

LRRFIP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: 293T, A431, NCI-H929

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of LRRFIP1 in whole cell lysate using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with LRRFIP1 polyclonal antibody (Product # PA5-78282) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of LRRFIP1 in whole cell lysate using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with LRRFIP1 polyclonal antibody (Product # PA5-78282) using a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of LRRFIP1 was performed by separating 30 µg of whole cell extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a LRRFIP1 Polyclonal Antibody (Product # PA5-78282) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

LRRFIP1 Polyclonal Antibody detects LRRFIP1 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LRRFIP1 protein stained by LRRFIP1 Polyclonal Antibody (Product # PA5-78282) diluted at 1:500. Blue: Hoechst 33342 staining.