Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-78704 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal CDCA2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- Store at 2 - 8 C 1 year from date of receipt
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CDCA2 Antibody [NBP2-78704] - Whole cell lysate (50 ug) from K-562, HEK293T, and U2OS cells prepared using NETN lysis buffer. Using this antibody for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CDCA2 Antibody [NBP2-78704] - Detection of human CDCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: This anti-body was used for IP at 100 ul per reaction. For blotting immunoprecipitated CDCA2, This antibody was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.