H00011117-M01

antibody from Abnova Corporation

Targeting: EMILIN1 DKFZp586M121, gp115

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00011117-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00011117-M01, RRID:AB_518778

Product name

EMILIN1 monoclonal antibody (M01), clone 4A3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant EMILIN1.

Antigen sequence

MADLGATKDRIISEINRLQQEATEHATESEGRFRG
LEEGQAQAGQCPSLEGRLGRLEGVCERLDTVAGGL
QGLREGLSRHVAGLWAGLRETNTTSQMQAALLEKL
VGGQAGLGRRLGALNSSLQLLEDRLHQLSLKDLTG
EGTKGPAGEAGPPGPPGLQGPPGPAGPPGSPGKDG
QEGPIGPPGPQGEQGVEGAPAAPVPQVAFSAALSL
PRSEPGTVPFDRVLLNDGGYYDPETGVFTAPLAGR
YLLSAVLTGHRHEKVEAVLSRSNQGVARVDSGGYE
PEGLEKKPVAESQPSPGTLGVFSLILPLQAGDTVC
VDLVMGQLAHSEEPLTIFSGALLYGDPELEHA


Isotype

IgG

Antibody clone number

4A3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of EMILIN1 expression in transfected 293T cell line by EMILIN1 monoclonal antibody (M01), clone 4A3.Lane 1: EMILIN1 transfected lysate(35.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged EMILIN1 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol