PA5-92950

antibody from Invitrogen Antibodies

Targeting: PYGB

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-92950

Provider

Invitrogen Antibodies

Product name

GPBB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LKFSAFLEKE YKVKINPSSM FDVHVKRIHE YKRQLLNCLH VVTLYNRIKR DPAKAFVPRT VMIGGKAAPG YHMAKLIIKL VTSIGDVVNH DPVVGDRLKV IFLENYRVSL AEKVIPAADL SQQISTAGTE ASGTGNMKFM LNGALTIGTM DGANVEMAEE AGAENLFIFG LRVEDVEALD RKGYNAREYY DHLPELKQAV DQISSGFFSP KEPDCFKDIV NMLMHHDRFK VFADYEAYMQ CQAQVDQLYR NPKEWTKKVI RNIACSGKFS SDRTITEYAR EIWGVEPSDL QIPPPNIPRD; Positive Samples: BT-474, SW620, HT-29, HepG2, Mouse brain, Mouse heart, Mouse lung, Rat brain, Rat heart

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.8 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GPBB in extracts of various cell lines. Samples were incubated with GPBB Polyclonal antibody (Product # PA5-92950) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of MCF-7 cells using PYGB Polyclonal antibody (Product # PA5-92950). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GPBB was performed in MCF-7 cells using GPBB Polyclonal Antibody (Product # PA5-92950).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GPBB in PC-12 cells. Samples were incubated with GPBB Polyclonal antibody (Product # PA5-92950) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GPBB in PC-3 cells. Samples were incubated with GPBB Polyclonal antibody (Product # PA5-92950) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GPBB in NIH/3T3 cells. Samples were incubated with GPBB Polyclonal antibody (Product # PA5-92950) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GPBB in HepG2 cells. Samples were incubated with GPBB Polyclonal antibody (Product # PA5-92950) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of GPBB in 200 μg extracts of HepG2 cells. Samples were precipitated with 3 μg GPBB Polyclonal antibody (Product # PA5-92950). Western blot was performed from the immunoprecipitate using GPBB Polyclonal antibody (Product # PA5-92950) at a dilution of 1:1,000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of GPBB was performed in 200 µg extracts of HepG2 cells using GPBB Polyclonal Antibody (Product # PA5-92950). Western blot was performed from the immunoprecipitate using GPBB Polyclonal Antibody.