Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [6]
- Immunoprecipitation [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-92950 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPBB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LKFSAFLEKE YKVKINPSSM FDVHVKRIHE YKRQLLNCLH VVTLYNRIKR DPAKAFVPRT VMIGGKAAPG YHMAKLIIKL VTSIGDVVNH DPVVGDRLKV IFLENYRVSL AEKVIPAADL SQQISTAGTE ASGTGNMKFM LNGALTIGTM DGANVEMAEE AGAENLFIFG LRVEDVEALD RKGYNAREYY DHLPELKQAV DQISSGFFSP KEPDCFKDIV NMLMHHDRFK VFADYEAYMQ CQAQVDQLYR NPKEWTKKVI RNIACSGKFS SDRTITEYAR EIWGVEPSDL QIPPPNIPRD; Positive Samples: BT-474, SW620, HT-29, HepG2, Mouse brain, Mouse heart, Mouse lung, Rat brain, Rat heart
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GPBB in extracts of various cell lines. Samples were incubated with GPBB Polyclonal antibody (Product # PA5-92950) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of MCF-7 cells using PYGB Polyclonal antibody (Product # PA5-92950). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GPBB was performed in MCF-7 cells using GPBB Polyclonal Antibody (Product # PA5-92950).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of GPBB in PC-12 cells. Samples were incubated with GPBB Polyclonal antibody (Product # PA5-92950) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of GPBB in PC-3 cells. Samples were incubated with GPBB Polyclonal antibody (Product # PA5-92950) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of GPBB in NIH/3T3 cells. Samples were incubated with GPBB Polyclonal antibody (Product # PA5-92950) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of GPBB in HepG2 cells. Samples were incubated with GPBB Polyclonal antibody (Product # PA5-92950) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of GPBB in 200 μg extracts of HepG2 cells. Samples were precipitated with 3 μg GPBB Polyclonal antibody (Product # PA5-92950). Western blot was performed from the immunoprecipitate using GPBB Polyclonal antibody (Product # PA5-92950) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of GPBB was performed in 200 µg extracts of HepG2 cells using GPBB Polyclonal Antibody (Product # PA5-92950). Western blot was performed from the immunoprecipitate using GPBB Polyclonal Antibody.