PA5-28022

antibody from Invitrogen Antibodies

Targeting: PYGB

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28022

Provider

Invitrogen Antibodies

Product name

GPBB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain. Predicted reactivity: Mouse (92%), Rat (91%), Zebrafish (85%), Chicken (86%), Sheep (92%), Rhesus Monkey (98%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.36 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Breast cancers utilize hypoxic glycogen stores via PYGB, the brain isoform of glycogen phosphorylase, to promote metastatic phenotypes.

Altemus MA, Goo LE, Little AC, Yates JA, Cheriyan HG, Wu ZF, Merajver SD
PloS one 2019;14(9):e0220973

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

GPBB Polyclonal Antibody detects GPBB protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. GPBB Polyclonal Antibody (Product # PA5-28022) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

GPBB Polyclonal Antibody detects PYGB protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. GPBB Polyclonal Antibody (Product # PA5-28022) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 3 Glycogen phosphorylase brain isoform knockdown inhibits glycogen utilization in breast cancer cells. (A) Western blot of liver and brain glycogen phosphorylase (PYGL and PYGB) in wild-type, either empty vector, shPYGL and shPYGB transduced MDA-MB-231, MCF-7, and MCF-10A cells. Actin is shown as a loading control(B-D) Glycogen levels normalized to protein in lysate after 24h in hypoxia (Hypoxic Control) or 24h in hypoxia with subsequent 24h in normoxia (Normoxic Exposure) for wild-type, empty vector, shPYGL, and shPYGB in (B) MDA-MB-231, (C) MCF-7, and (D) MCF-10A cells, respectively. Error bars represent SEM of n = 3. *p