PA5-20812

antibody from Invitrogen Antibodies

Targeting: AGTR1 AG2S, AGTR1A, AGTR1B, AT1, AT1B, AT2R1, AT2R1A, AT2R1B, HAT1R

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20812

Provider

Invitrogen Antibodies

Product name

AGTR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is mouse kidney tissue lysate.

Concentration

1 mg/mL

References

Submitted references

Myogenic Vasoconstriction Requires Canonical G(q/11) Signaling of the Angiotensin II Type 1 Receptor.

Cui Y, Kassmann M, Nickel S, Zhang C, Alenina N, Anistan YM, Schleifenbaum J, Bader M, Welsh DG, Huang Y, Gollasch M
Journal of the American Heart Association 2022 Feb 15;11(4):e022070

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Effect of Angiotensin-Converting-Enzyme Inhibitor and Angiotensin II Receptor Antagonist Treatment on ACE2 Expression and SARS-CoV-2 Replication in Primary Airway Epithelial Cells.

Okoloko O, Vanderwall ER, Rich LM, White MP, Reeves SR, Harrington WE, Barrow KA, Debley JS
Frontiers in pharmacology 2021;12:765951

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Spent Hen Muscle Protein-Derived RAS Regulating Peptides Show Antioxidant Activity in Vascular Cells.

Fan H, Bhullar KS, Wu J
Antioxidants (Basel, Switzerland) 2021 Feb 15;10(2)

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Effect of (-)-epicatechin on the modulation of progression markers of chronic renal damage in a 5/6 nephrectomy experimental model.

Montes-Rivera J, Arellano-Mendoza M, Nájera N, Del Valle-Mondragón L, Villarreal F, Rubio-Gayosso I, Perez-Duran J, Meaney E, Ceballos G
Heliyon 2019 Apr;5(4):e01512

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Data supporting that adipose-derived mesenchymal stem/stromal cells express angiotensin II receptors in situ and in vitro.

Ageeva LV, Sysoeva VY, Tyurin-Kuzmin PA, Sharonov GV, Dyikanov DT, Stambolsky DV, Kalinina NI
Data in brief 2018 Feb;16:327-333

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Differential expression and DNA methylation of angiotensin type 1A receptors in vascular tissues during genetic hypertension development.

Pei F, Wang X, Yue R, Chen C, Huang J, Huang J, Li X, Zeng C
Molecular and cellular biochemistry 2015 Apr;402(1-2):1-8

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse kidney tissue lysate using a AGTR1 polyclonal antibody (Product # PA5-20812) at (A) 1 and (B) 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of AGTR1 in mouse kidney tissue lysate with AGTR1 Polyclonal Antibody (Product # PA5-20812) at (A) 1 and (B) 2 µg/mL.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of mouse kidney cells using a AGTR1 polyclonal antibody (Product # PA5-20812) at a 20 µg/mL dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of AGTR1 in Mouse Kidney cells with AGTR1 Polyclonal Antibody (Product # PA5-20812) at 20 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of AGTR1 in mouse kidney tissue with AGTR1 Polyclonal Antibody (Product # PA5-20812) at 2.5 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of AGTR1 in mouse kidney tissue with AGTR1 Polyclonal Antibody (Product # PA5-20812) at 2.5 µg/mL.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 Effect of VRP, LKY, VRY, and V-F on the expressions of AT 1 R ( A ) and MasR ( B ) in VSMCs. Cells were treated with peptides (50 muM) for 24 h. Protein bands were quantified by densitometry and normalized to alpha-tubulin. Data were expressed as means +- SEM of 4-5 independent experiments, normalized to the untreated group (Untr); ns: not significant ( p > 0.05); * p < 0.05, as compared to the untreated group.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 7 Immunofluorescence staining for ATR1 and ACE2 in primary differentiated airway epithelial cell cultures infected with SARS-CoV2 and treated with either captopril or losartan. Cultures were infected with SARS-CoV-2 for 96 h and a subset were treated with captopril (1 uM) or losartan (2 uM) throughout the 96-h infection. Depicted are representative confocal images obtained through the apical plane of differentiated airway epithelial cultures; ATR1 staining is shown in green (A) , ACE2 staining is shown in red (B) , and merged images including DAPI staining (blue) are shown in the (C) . No significant differences in staining pattern were observed between the groups (63x objective; Scale bars = 50 um).